Determination of cross-sterile groups in a polymorphic fern Ceratopteris thalictroides

多态性蕨类植物角蕨交叉不育群的测定

• 批准号: 06640909
• 负责人: MASUYAMA Shigeo
• 金额: $ 0.51万
• 依托单位: Tokyo Woman's Christian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06640909/
• 关键词: Allozyme; Artificial hybridization; Ceratopteris thalictroides; Fern; Reproductive isolation; 交配群

In 1994 to 1995, available spores were obtained from the following 11 localities : three localities in Taiwan, one in India, three in Indonesia, one in New Guinea, one in Hawaii of USA,and each one in Surinum and Guiana in South America. Spores from these localitiesand those from Okinawa Prefecture and Chiba Prefecture, which were selected as the representatives of the south type and the north type of C.thalictroides, respectively, were sown onto agar media to yield gametophytes. Developed gametophytes were conducted to undergo self-fertilization within each source and crossing between two Japanese sources and eleven foreign sources. Electrophoretic analyzes on PGI and PGM allozymes were carried out for sporophyte progeny. Sporophyte progeny of 196 offsprings were obtained through crossing treatment. Out of these, 60 offsprings were impossible to be judged as selfed ones or hybrids, because parental sporophytes had the same allozymes as the north type and the south type. Allozyme analyzes revealed that, of remaining 136 offsprings, 74 were of selfed origin and 62 were of hybrid origin. Spores of hybrid sporophytes were sown onto agar media. The degree of the germination rate of spores indicated that one spore source in Indonesia was of the north type and the other two were probably of the south type. It was also indicated that all the three spore sources of Taiwan were of the north type and the spore source of Guiana of South America was probably of the south type. Whether the remaining hybrids are of the north type or the south type will be checked soon after they will produce spores. The present study suggests that there are at least two cross-sterile groups in C.thalictroides in the world.

1994 ~ 1995年，从台湾、印度、印度尼西亚、新几内亚、美国夏威夷、南美洲的苏里南和圭亚那等11个地区采集到有效孢子。将这些地区的孢子以及分别作为南型和北型代表的冲绳县和千叶县的孢子接种到琼脂培养基上，获得配子体。对发育的配子体进行每个来源内的自花受精和两个日本来源和11个外国来源之间的杂交。对孢子体后代进行了PGI和PGM等位酶的电泳分析。通过杂交处理获得196个后代的孢子体后代。其中有60个后代不能判断为自交后代或杂种，因为亲本孢子体具有与北方型和南方型相同的等位酶。等位酶分析表明，其余136个后代，74个是自交起源和62个是杂交起源。将杂交孢子体的孢子播种在琼脂培养基上。孢子萌发率的高低表明，印度尼西亚的一个孢子源属于北方型，另外两个孢子源可能属于南方型。台湾的三个孢子源均为北方型，南美洲圭亚那的孢子源可能为南方型。剩下的杂种是北方型还是南方型，在它们产生孢子后不久就要检查。本研究表明，世界上至少存在两个杂交不育群。