Molecular shape recognition by poly (gamma-methyl L-glutamate) spherical particles with secondary structures

具有二级结构的聚(γ-甲基L-谷氨酸)球形颗粒的分子形状识别

• 批准号: 06651032
• 负责人: HIRAYAMA Chuichi
• 金额: $ 1.28万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06651032/
• 关键词: L-glutamate; spherical particle; alpha-helix; beta-structure; secondary structure; molecular shape recognition; cytochrome C; endotoxin; L-グルタミン酸; PMLG粒子; リッキドクロユトグラフィー; ミクロスフェア; アフィニティ

We report the preparation of PMLG particles with alpha-helixes and beta-structures and their characteristics. We report also the molecular shape recognition for geometrical isomers of hexanol using PMLG particles and endotoxin-adsorption using aminated PMLG particles. The results can be summarized as follows :1) Porous poly (gamma-methyl L-glutamate) spherical particles underwent alpha-helix-to-beta-structure transition in the main chain as a result of heat treatment in water or methanol. The particles obtained maintained their sphericity and characteristic porosity after the transition, while the transition increased insolubility against organic solvents due to intermolecular hydrogen bonding.2) Adsorption capacity of the PMLG particles for cytochrome C depended remarkably on the content of beta-structure as well as the amount of carboxyl groups introduced.3) PMLG particles which the main chain formed alpha-helixes showed molecular shape recognition for hexanol isomers in liquid chromatography process.4) Aminated PMLG particles with alpha-helixes selectively removed endotoxin (lipopolysaccharide) from an endotoxin-protein solution without affecting the recovery of the protein.

我们报道了具有α -螺旋和β -结构的PMLG粒子的制备及其特性。我们还报道了使用PMLG颗粒对己醇几何异构体的分子形状识别和使用胺化PMLG颗粒对内毒素的吸附。结果表明：1)多孔聚（γ -甲基l -谷氨酸）球形颗粒经水或甲醇热处理后，主链发生了α -螺旋结构向β -结构的转变。所获得的颗粒在转变后保持了其球形和特征孔隙度，但由于分子间氢键的作用，转变增加了对有机溶剂的不溶解度。2) PMLG颗粒对细胞色素C的吸附能力与β -结构的含量和引入羧基的量有显著关系。3)主链形成α -螺旋的PMLG颗粒在液相色谱过程中对己醇异构体具有分子形状识别能力。4)具有α -螺旋的氨基化PMLG颗粒选择性地从内毒素蛋白溶液中去除内毒素（脂多糖），而不影响蛋白质的回收。

项目成果

平山忠一: "The removal of endotoxins from protein solutions using column packings with aminated poly(γ-methyl L-glutamate)spheres" Chromatographia. 40. 33-38 (1995)

Chuichi Hirayama：“使用胺化聚（γ-甲基 L-谷氨酸）球体柱填料去除蛋白质溶液中的内毒素”色谱图 40. 33-38 (1995)。

平山忠一: "Preparation of porous spherical poly(γ-methyl L-glutamate)particles containing β-structue" Polymer Journal. 26. 61-66 (1994)

Chuichi Hirayama：“含有β结构的多孔球形聚(γ-甲基L-谷氨酸)颗粒的制备”Polymer Journal 26. 61-66 (1994)。

Chuichi Hirayama, Hirotaka lhara, Shin-Ichiro Ishihara, Yukio Imanishi: "Preparation of porous poly (gamma-methyl L-glutamate) particles containing beta-structure" Polymer Journal. Vol.26. 61-66 (1994)

Chuichi Hirayama、Hirotaka lhara、Shin-Ichiro Ishihara、Yukio Imanishi：“含有 β 结构的多孔聚（γ-甲基 L-谷氨酸）颗粒的制备”聚合物杂志。

伊原博隆: "配向性分子材料の機能設計と用途新展開" アイ・ピ-・シ-. 1-288 (1995)

Hirotaka Ihara：“定向分子材料的功能设计和新应用开发”IPC 1-288 (1995)。

Chuichi Hirayama, Masayo Sakata, Sunao Morimoto, Hiroshi Tachibe, Hirotaka Ihara, Kunio Ohkuma, Yasuyoshi Miyachi: "The removal of endotoxins from protein solutions using column packings with aminated poly (gamma-methyl L-glutamate) spheres" Chromatograph

Chuichi Hirayyama、Masayo Sakata、Sunao Morimoto、Hiroshi Tachibe、Hirotaka Ihara、Kunio Ohkuma、Yasuyoshi Miyachi：“使用胺化聚（γ-甲基 L-谷氨酸）球体柱填料去除蛋白质溶液中的内毒素”色谱仪