Polypeptide Microshpheres

多肽微球

• 批准号: 63550689
• 负责人: HIRAYAMA Chuichi
• 金额: $ 1.22万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63550689/
• 关键词: porous; sphere; poly (gamma-methyl L-glutamate); chromatography; affinity; latex particle

Porous and spherical particles of poly (gamma-methyl L-glutamate) were prepared by our unique method. The diameter of spheres was controlled by adjusting the suspension condition, for example the concentration of poly(vinyl alcohol) as a suspension medium and poly (gamma-methyl L-glutamate), stirring spped and suspension temperature. As a result, we obtained poly (gamma-methyl L-glutamate) spheres with average diameters of 0.1 mum to 1000 mum The macroreticulation of spheres was achieved by use of diluent in the process of sphering. Hydrophobic solvents with partial polar groups were effective to make macroporous, for example decahydronaphthalene, dialkylphthalate and oleic acid. The secondary structures of peptide chains in the spheres was determined. The content of secondary structures is adjustable by incubation of material solution and treatment of formic acid. The affinity of spheress for proteins were examined by a column method. The spheres have specific affinity for proteins. This is in agreement with the result presented here, where transformation incidences were reduced in the presence of PKC activators.

用我们独特的方法制备了多孔球形聚谷氨酸甲酯L。通过调整悬浮条件，如聚乙烯醇作为悬浮介质和聚谷氨酸甲酯的浓度、搅拌速度和悬浮温度来控制微球的直径。结果制得了平均粒径为0.1um~1000um的聚谷氨酸甲酯-L微球。在球化过程中使用稀释剂实现了微球的大网状化。含有部分极性基团的疏水溶剂可以有效地制备大孔，如十氢对苯二甲酸、邻苯二甲酸二烷基和油酸。测定了球内多肽链的二级结构。通过原料溶液的孵化和甲酸的处理，可以调节二级结构的含量。用柱状法测定了蛋白质的球形性。这些球体对蛋白质有特殊的亲和力。这与这里给出的结果是一致的，在PKC激活剂的存在下，转化发生率降低了。

项目成果

伊原 博隆: "“Macroreticulation of spherical poly(γーmethy Lーglutamate) beads"" Journal of Applied polymer Science.

Hirotaka Ihara：“球形聚（γ-甲基L-谷氨酸）珠的宏观网状结构”，《应用高分子科学杂志》。

伊原博隆: "“Macroreticulation of spherical poly(γ-methyl L-glutamate)beads"" Journal of Applied Polymer Science.

Hirotaka Ihara：“球形聚（γ-甲基L-谷氨酸）珠的宏观网状结构”，《应用高分子科学杂志》。

平山忠一: "“Regulation of pore size distribution of poly(γ-methyl L-glutamate)spheres as a gel permiation chromatography packing"" Polymer Journal.

Chuichi Hirayama：“作为凝胶渗透色谱填料的聚（γ-甲基L-谷氨酸）球体孔径分布的调节”《聚合物杂志》。

平山忠一: Journal of Chromatography.

平山中一：色谱杂志。

平山忠一、伊原博隆、遠藤剛 編: "アミノ酸ポリマー" (株)シー・エム・シー, 236-259 (1988)

平山中一、伊原广隆、远藤刚编：“氨基酸聚合物” CMC Co., Ltd., 236-259 (1988)