Fundamental Study on Bacterial Cellulose Production

细菌纤维素生产的基础研究

• 批准号: 06660216
• 负责人: SAMESHIMA Kazuhiko
• 金额: $ 1.22万
• 依托单位: KOCHI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660216/
• 关键词: cellulose; bacterial cellulose; lignosulfonate; Acetobacter xylinum; fibril; フィブリル; リグニン; ミクロフィブリル; 生合成

The effect of lignosulfonate on the bacterial cellulose production has been studied for two years and the following are the results ;(1) The relationship between the high cellulose yield increase and the molecular weight of lignosulfonate was investigated through the fractionation of a lignosulfonate sample by the ethanol-water precipitation method. The result showed relatively high molecular weight fraction is effective.(2) The yield increase by lignosulfonate addition to the medium was confirmed with various stock cultures and carbon sources. The effect of the lignosulfonate had so-called universality.(3) The observation through the transmit electron microscopy showed that the fibril formation by the lignosulfonate added medium is as normal as the standard medium, and the yield increase effect of lignosulfonate occured without any interference to the micro-fibril formation.Above resutlts indicate that the reasearch on the mechanism of the high yield increase of lignosulfonate addition will lead to a fundamental condition for the commercial production of bacterial cellulose. The screening of bacteria with a high cellulose procuction ability might be one of other essentials for the commercial use.

本文研究了木质素磺酸盐对细菌纤维素产量的影响，结果如下：（1）通过乙醇-水沉淀法分离木质素磺酸盐样品，研究了木质素磺酸盐的分子量与纤维素产量的关系。结果表明，相对高分子质量组分是有效的。(2)通过木质素磺酸盐添加到培养基中的产量增加用各种储备培养物和碳源证实。木质素磺酸盐的作用具有普遍性。(3)通过透射电子显微镜的观察表明，通过添加木质素磺酸盐的培养基的原纤形成与标准培养基一样正常，木质素磺酸盐的增产效果对微生物没有任何干扰，以上结果表明，对木质素磺酸盐高产机理的重新认识，将为细菌纤维素的工业化生产提供基础条件纤维素.筛选高纤维素生产能力的细菌可能是商业用途的其他要素之一。