PRODUCTION,CUMULATIVE PATTERN AND UTILIZATION OF PROTEIN FRACTIONATED IN TROPICAL FORAGE SPECIES.

热带饲料品种中蛋白质分级的生产、累积模式和利用。

• 批准号: 06660340
• 负责人: KAWAMOTO Yasuhiro
• 金额: $ 1.34万
• 依托单位: UNIVERSITY OF THE RYUKYUS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660340/
• 关键词: TROPICAL GRASS; TROPICAL LEGUME; FEED PROTEIN; SILAGE; DEGRADABLE PROTEIN; INDIGESTIBLE PROTEIN; HEATING; 不消化タンパク質

This study was firstly conducted to establish a suitable methods to evaluate dietary protein in terms of meeting ruminants, and compare a dietary protein fractionated based on solubility in each detergent solution between some tropical forages. Secondly, the effects of heating during silage fermentation and secondary fermentation after ensiling on protein fractions and degradation in rumen of some tropical forage silages were estimated. Results were as follows.1) When applying the methods in this study, dietary protein system s were distinguished a partition rumen degradable protein (nitrogen x 6.25) into quickly soluble and slowly degradated fractions and partition rumen undegradable protein into digestible and indigestible fractions. 2) The in situ and in vitro methods were introduced to measure an indigestible nitrogen in ruminants, which is characterized as acid detergent insoluble nitrogen (ADIN). These two new methods were significantly correlated with ADIN (r=0.7-0.9) and a reliable alternative to nitrogen after ADF analysis. 3) The degradable protein fraction in all the six tropical forage species were increased by ensiling, which tendency was remarkable in legumes. 4)The dry matter digestibility, degradated protein fraction were decreased, when silages were exposed to heat and moisture during and after ensiling, while indigestible nitrogen content were remarkably increased.

这项研究首先是为了建立一种在遇到反刍动物方面评估饮食蛋白的合适方法，并比较了一些热带草料之间的每种洗涤剂溶液中的溶解度分馏的饮食蛋白。其次，估计了一些热带草青贮饲料的蛋白质分数和蛋白质分数和降解后，在青贮发酵过程中加热和继发性发酵的影响。结果如下。1）当在本研究中应用这些方法时，将饮食蛋白系统S区分开，将瘤胃降解蛋白（氮X 6.25）分配为迅速溶解，并缓慢降解的分数和瘤胃不可促进的蛋白质不可促进蛋白变成可几乎可见的蛋白质。 2）引入了原位和体外方法，以测量反刍动物中不可消化的氮，该氮的特征在于酸洗涤剂不溶性氮（Adin）。这两种新方法与Adin（r = 0.7-0.9）显着相关，并且在ADF分析后是氮的可靠替代方法。 3）所有六种热带草料物种中可降解的蛋白质分数通过杀戮增加，这在豆类中是显着的。 4）干物质的消化率，降解的蛋白质分数减少，而在沉积期间和后期暴露于热量和水分时，同时含有可观的氮含量明显增加。

项目成果

Kawamoto,Y and E Miyagi: "Ruminal Protein Fractions in Dietary Tropical Pastune Legume" Proceeding of the 7th Asian-Anstralasian Animal Science Congnass.1. 43-44 (1994)

Kawamoto，Y 和 E Miyagi：“膳食热带 Pastune 豆类中的瘤胃蛋白组分”第七届亚洲-安斯特拉西亚动物科学会议论文集。1。

Kawamoto Y.and E.Miyagi: "Ruminal Protein Fractions in Dietary Tropical Pasture Legume" Proc.of the 7th Asian-Australasian Animal Science Congress. Vol 1. 43-44 (1994)

Kawamoto Y. 和 E.Miyagi：“膳食热带牧场豆类中的瘤胃蛋白组分”第七届亚洲-澳大利亚动物科学大会论文集。