Morphological studies of marginal nuclei in chicken spinal cord.

鸡脊髓边缘核的形态学研究。

• 批准号: 06660379
• 负责人: UEHARA Masato
• 金额: $ 1.34万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06660379/
• 关键词: Chicken; Marginal Nuclei; Spinal Cord; Structure; 神経核; 白質; グリコーゲン

Marginal nuclei (MN) consist of 3 subnuclei, major (Mj), minor (Mn) and ventral (V) marginal nuclei. We observed light and electron microscopically marginal nuclei. Mn were segmentally located in the lateral column. Mj consisted of the white matter and projected parts and the adjoining white matter parts overlaped partly in distribution. Neurons in Mn and Mj were medium in mean size and in V were large. Appearance of Nissle substances correlated with the size of neurons. Small-sized neurons included a few small clusters of nissle substance separated from one another. Cytoplasm of medium-sized neurons included plenty of rER and free ribosomes that consist of Nissle substance but the Nissle substance did not form cluster or block. In large-sized neurons Nissle substance had the form of large blocks separated from one another.Presynaptic boutons on their somata and dendrites of marginal nuclei neurons were divided according to the shape of synaptic vesicles. There were 4 types of presynap … More tic boutons, S type characterized by clear and spherical vesicles, SG type characterized by clear and spherical vesicles plus granular vesicles, SF type characterized by clear and spherical vesicles plus clear and flattened vesicles, and SFG type characterized by the above mentioned 3 types of vesicles. S type occurred most frequently. There were not significant differences in the frequency of these type of presynaptic boutons among the subnuclei.Based on the morphology, we suggested that Mn and Mj may be the same functional significance but different from V.The white matter and projected parts of Mj may be the same functional significance.Astrocytes in MN had pecliar structures. Mj and V included a large amount of glycogen containing astrocytes. These glycogen containing astrocytes ranged in structure from glycogen body cells to fibrous astrocytes. Astorocytes with a few processes and markedly dilated cisterns of rER distributed in Mn and Mj. Although Mn appeared to be gray matter, astrocytes in Mn were fibrous type.Axons of MN neurons in the lumbosacral intumescence terminated in the rostral various levels below the cervical intumescence and axons of MN neurons in the cervical one terminated in the various caudal levels above the lumbosacral one Less

边缘核（MN）由3个亚核组成：主核（Mj）、次核（MN）和腹侧核(V)。我们在光镜和电子显微镜下观察到边缘核。Mn呈节段性分布于侧柱。Mj由白质和突起部分组成，相邻的白质部分在分布上部分重叠。Mn和Mj组神经元平均大小中等，V组神经元平均大小较大。鼻物质的出现与神经元的大小有关。小尺寸的神经元包括一些彼此分离的小簇鼻状物质。中等大小的神经元细胞质中含有大量的内质网和游离核糖体，这些核糖体由尼索物质组成，但尼索物质不形成簇状或块状。在大尺寸的神经元中，尼索物质具有彼此分离的大块的形式。突触前钮扣和边缘核神经元树突根据突触囊泡的形状划分。突触前有4种类型，其中以清晰球形囊泡为特征的S型，以清晰球形囊泡+颗粒状囊泡为特征的SG型，以清晰球形囊泡+清晰扁平囊泡为特征的SF型，以上述3种囊泡为特征的SFG型。S型发生频率最高。这些类型的突触前钮扣的频率在亚核之间没有显著差异。根据形态学分析，我们认为Mn和Mj可能具有相同的功能意义，但与v不同，Mj的白质和突起部分可能具有相同的功能意义。MN的星形胶质细胞结构特殊。Mj和V含有大量含糖原的星形胶质细胞。这些含糖原的星形胶质细胞在结构上从糖原体细胞到纤维状星形胶质细胞不等。星形胶质细胞突起较少，内质网池明显扩张，分布于Mn和Mj。虽然Mn表现为灰质，但Mn的星形胶质细胞为纤维型。腰骶部肿胀区MN神经元轴突终止于颈部肿胀以下的吻侧不同水平，颈部肿胀区MN神经元轴突终止于腰骶部肿胀以上的尾侧不同水平