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Role of central vasopressin in cardiovascular regulation in rats

中枢加压素在大鼠心血管调节中的作用

基本信息

批准号：
06670130
负责人：
TAKANO Yukio
金额：
$ 1.47万
依托单位：
Fukuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670130/
关键词：
Vasopressin Vasopressin receptor Baroreceptor reflex Hypertension V1a receptor mRNA V2 receptor mRNA Area postrema 最後野抗利尿作用 V_1受容体mRNA V_2受容体mRNA 受容体拮抗薬海馬

项目摘要

1. Vasopressin (AVP) receptor subtypes in rat brain :To determine the subtype (s) of the AVP receptor in the brain, we examined the inhibitions of specific binding of [^3H] -AVP by the V1 antagonist OPC-21268 and V2 antagonist OPC-31260. [^3H] -AVP binding to rat hippocampal membranes was strongly inhibited by the V1 antagonist, OPC-21268. In this study, we tested for AVP V1a-and V2-receptor mRNAs in the hippocampus of adult rats by a reverse transcription-polymerase chain reaction (RT-PCR) method. PCR product of the V2 receptor mRNA was detected in the hippocampus, but that of V1a receptor mRNA was not. We do not know why V1a receptor mRNA was not detected in the hippocampus in this study. Possible explanations are that the sequence of the AVP receptor mRNA in the brain may not completely consistent with that of V1a receptor mRNA in peripheral tissues.2.Role of vasopressin in the area postrema in regulation of baroreceptor reflex.We examined the involvements of vasopressin and its receptors in the sensitivity of the baroreceptor reflex in hypertension. The sensitivity of baroreceptor reflex was remarkably increased via V2 receptor in the area postrema in the initial stage of one-kidney, one clip hypertensive rats, but not in the other hypertensive models. And this period, V2 receptor mRNA was expressed temporarily at the area postrema. These results suggested that vasopressin in the area postrema have very important roles of baroreceptor reflex and development of a kind of hypertension.

1.大鼠大脑中的加压素（AVP）受体亚型：为了确定大脑中AVP受体的亚型，我们检查了V1拮抗剂OPC-21268和V2拮抗剂OPC-31260对[^3H]-AVP特异性结合的抑制作用。 [^3H]-AVP 与大鼠海马膜的结合受到 V1 拮抗剂 OPC-21268 的强烈抑制。在这项研究中，我们通过逆转录聚合酶链反应 (RT-PCR) 方法测试了成年大鼠海马中的 AVP V1a 和 V2 受体 mRNA。在海马中检测到V2受体mRNA的PCR产物，但未检测到V1a受体mRNA的PCR产物。我们不知道为什么本研究中在海马中没有检测到 V1a 受体 mRNA。可能的解释是大脑中AVP受体mRNA的序列可能与外周组织中V1a受体mRNA的序列不完全一致。2.后区加压素在调节压力感受器反射中的作用。我们研究了加压素及其受体在高血压压力感受器反射敏感性中的作用。单肾一夹高血压大鼠初始阶段通过后区V2受体使压力感受器反射的敏感性显着增加，而在其他高血压模型中则没有显着增加压力感受器反射的敏感性。而这一时期，V2受体mRNA在后区暂时表达。这些结果表明，后区加压素对于压力感受器反射和一种高血压的发生具有非常重要的作用。