Pathology Analysis of Prolifecation and Metastasis in Prostatic Carcinoma

1)应用计算机图像分析系统对前列腺癌组织中雄激素受体(AR)、增殖细胞标志物(Ki-67)与组织学分级的关系进行了研究。前列腺癌组织学分级与AR、Ki-67显著相关，而临床分期与AR、Ki-67无关。用双抗体夹心ELISA法测定了神经内分泌肿瘤(Net)、前列腺癌(PC)和良性前列腺增生症(BPE)患者血浆嗜铬粒蛋白A(CgA)水平，作为内分泌分化的指标。BPH组与Net组、PC组与Net组比较，血浆CgA水平差异均有统计学意义(P<0.001)。在33例前列腺癌患者中，5例CgA升高，其中仅1例PSA升高。我们认为CgA是Net的一个很好的标记物，在PSA未升高的患者中，CgA的检测也有助于发现前列腺癌。3)我们分析了50例前列腺癌中的生长因子：IGF-1、EGFR、BMP2和TIMP-II。IGF-I在肿瘤细胞和间质细胞中均有表达。EGFR仅在上皮细胞中表达，尤其在基底细胞中表达较强。BMP2和TIMP-II仅在基质细胞中表达。综上所述，上述生长因子均介导前列腺癌的生长。

1) We investigated corrlation of androgen receptor (AR) , proliferating cell marker (Ki-67) and histologic grade in prostatic carcinoma using a computer-assisted image analysis system. There was a significant correlation between the histologic grade, and AR and Ki-67 ; however, there was no correlation between the clinical stage and AR or Ki-67.2) Endocrine differentiation in prostatic carcinoma cells has been considered one of the causes of worse prognosis because of unresponsiveness for hormone therapy. We measured plasma levels of chromogranin A (CgA) by ELISA as an indicator of endocrine differentiation in patients with neuroendocrine tumors (NET), and prostatic carcinoma (PC) and benign prostatic hyperplasia (BPE). There were significant differences between plasma levels of CgA in BPH and NET (P<0.001), and between the levels in PC and NET (P<0.01). of the 33 patients with prostatic carcinoma, five had elevated CgA in which only one had elevated PSA.We concluded that CgA is an excellent marker for NET,and measurement of CgA is also useful to detect prostatic carcinoma in patients whose PSA is not elevated.3) We analyzed growth factors ; IGF-1, EGFR,BMP2, and TIMP-II in prostatic carcinomas in 50 prostatic carcinomas. IGF-I was detected in both tumor cells and stromal cells. EGFR was detected only in the epithelial cells, especially strong in the basal cells. BMP2 and TIMP-II were detected in only the stromal cells. In conclusion, all above growth factors mediate growth of prostatic carcinoma.