Molecular biological study on hormone production and tumor growth in endocrine tumor.

这个项目的重点是揭示我。肿瘤生长，和II.激素的产生，如1）高度恶性肿瘤和低度恶性肿瘤的生物学特征，2）肿瘤中多种激素产生的机制，3）利用分子生物学技术研究功能性肿瘤和非功能性肿瘤的差异。肿瘤生长：1.在正常胰腺腺泡细胞中检测到人再生基因（reg）的mRNA和reg蛋白。Reg被认为是胰腺腺泡细胞肿瘤的可靠标志物，但不能作为内分泌肿瘤的标志物.一个比较。对神经内分泌腺瘤（NEC）和类癌研究表明，PCNA阳性肿瘤细胞在NEC中的频率较高，而在类癌中的频率较低; HLA-DR抗原阳性基质细胞在NEG中较少，而在类癌中明显。PCNA和HLA-DR抗原可能是肿瘤生长的重要因素。激素产生：1.人神经母细胞瘤细胞株（NB-1）经cAMP和佛波酯处理后表达多种激素。VIP、PHM、神经肽Y、生长抑素、甲硫氨酸脑啡肽和酪氨酸羟化酶（TH）等基因.原位杂交和免疫组化检测显示，伴WDHA综合征的神经节神经母细胞瘤表达VIP、PHM、生长抑素、甲硫脑啡肽、神经肽Y、促肾上腺皮质激素释放肽（CRP）和TH等多种激素基因.对功能性和非功能性嗜铬细胞瘤中的儿茶酚胺合成酶进行免疫组化检测，发现部分非功能性肾上腺外副神经节瘤缺乏TH。对神经内分泌肿瘤中嗜铬粒蛋白、突触素和中间丝进行免疫组化研究.研究了神经内分泌肿瘤中免疫反应性芳香族L-氨基酸脱羧酶。

This project was focused on revealing I. Tumor growth, and II. Hormone production such as 1)bistological features between highly malignant and low grade malignant tumors, 2)the mechanism of multiple hormone production in the tumors and 3)the differences between functioning and non-functioning tumors using molecular biological techniques.I. Tumor growth :1. Messenger RNA of human regenerating gene(reg)and reg protein were detected in acinar cells of the normal pancreas. Reg was considered to be a : reliable marker for acinar cell tumors of the pancreas, but not for endocrine tumors.2. A comparative. study of neuroendocrine barcinoma(NEC)and carcinoid tumors revealed that frequency of PCNA positive tumor cells are high in NEC but low in carcinoids, and HLA-DR antigen positive stromal cells are few in NEG but evident in carcinoids. Both PCNA and HLA-DR antigen seemed to be important factors on tumor growth.II. Hormone production :1. Human neuroblastoma cell line(NB-l)treated with CAMP and phorbol ester expressed iffultiple hormone. genes such as VIP, PHM, neuropeptide Y, somatostatin, met-enkephalin and tyrosine hydroxylase(TH), using in situ hybridization, imunocytochemistry and radioimmunoassay.2. Ganglioneuroblastomas with WDHA syndrome expressed multiple hormone genes such as VIP, PHM, somatostatin, met-enkephalin, neuropeptide Y, corticotropin releasing peptide(CRP)and TH using in situ hybridization and imunohistochemistry.3. Catecholamine synthesizing enzymes in functioning and non-functioning pheochromocytomas were immunohistochemically examined and it revealed that some of non-functioning extra-adrenal paragangliomas lack TH.4. Immunohistochemical studies of chromogranin, synptophysin and intermediate filaments in neuroendocrine tumors were performed.5. Immunoreactive aromatic L-amino acid decarboxylase in neuroendocrine tumors was investigated.