Immunologic significance of intraepithelial lymphocytes in human palatine tonsils : Morphofunctional approach using immunoelectron microscopy and labeled antigen method

覆盖人腭扁桃体的网状黏膜以以b细胞和浆细胞为主的单核细胞密集浸润和毛细血管网络的存在为特征。以含IgG为主的浆细胞分布在网状黏膜的基底部。粘膜中可见4种b细胞，分别为1)bcl-2阳性和CD10/ cd23阴性的“边缘区”型，2)sIgD/CD5/ cd24阳性的“套区”型，3)CD10/ dna7阳性的“生发中心”型，4)cd11b阳性和cd16阴性的“B-1”型。免疫电镜双染法鉴定cd5阳性b细胞。至于t细胞，cd4阳性的T_H细胞在粘膜中占主导地位，其中分布着少量推测为CD56和tcrγ - δ阳性的胸腺外t细胞。系统发育上的老淋巴样细胞如“B-1”型b细胞和“胸腺外”t细胞的功能意义需要进一步的研究来阐明。扁桃体网状黏膜与胸腺髓质在形态功能上的相似性值得注意。在为“标记抗原法”制备mbp标记融合蛋白以证明细胞产生流感嗜血杆菌特异性抗体时遇到了困难。使用细菌DNA随机标记是不成功的。然后，我们尝试用PCR方法制备含有流感嗜血杆菌特异性蛋白片段（如蛋白D和脂肪酸结合蛋白）的重组蛋白。但是，目前我们还没有取得令人满意的结果。我们将继续为建立标记探针的方法而努力。

The reticular mucosa covering the human palatine tonsils was characterized by the dense infiltration of mononuclear cells mainly consisting of B-cells and plasma cells and by the presence of the capillary network. The plasma cells predominantly containing IgG were distributed in the basal half of the reticular mucosa. Four kinds of B-cells were observed in the mucosa, including 1) bcl-2-positive and CD10/CD23-negative "marginal zone" -type, 2) sIgD/CD5/CD24-positive "mantle zone" type, 3) CD10/DNA7-positive "germinal center" type, and 4) CD11b-positive and CD16-negative "B-1" type. CD5-positive-B-cells were identified by double staining in immunoelectron microscopy. As for T-cells, CD4-positive T_H cells predominated in the mucosa, where a few putative extrathymic T-cells positive for CD56 and TCRgammadelta were distributed. Furtherstudies are required to clarify the functional significance of the phylogenetically old lymphoid cells, such as "B-1" type B-cells and "extrathymic" T-cells. Morphofunctional similarities between the reticular mucosa of the tonsil and the medulla of the thymus were noteworthy.Difficulties were encountered in the preparation of MBP-labeled fusion proteins for the "labeled antigen method" to demonstrate cells producing antibodies specific for Hemophilus influenzae. Random-labeling using the bacterial DNA was unsuccessful. Then we tried to prepare recombinant proteins containing fragments of H.influenzae-specific proteins such as protein D and fatty acid-binding proteins by using a PCR method.However, we do not yet obtain satisfactory results at present. Continuous effort will be made for the establishment of the method for labeled probes for this new approach.