The significance of polyADP ribosylation in 'magentosis', a new type of cell death: Molecular pathologic approaches to magentosis in a regressing stage of human lactating breast and other hormone-responsive organs

品红是我们在人类哺乳期发现的一种新的细胞死亡模式，其组织化学特征是PAS阳性(品红色)细胞核中单链DNA的积聚。为了阐明洋红病的生物学意义，进行了以下实验。1)对不同的人类激素反应器官进行了显微筛选，以确定这一独特的现象。除1例甲状腺乳头状癌外，未见洋红色核。甲状腺癌中的肉芽肿病即将出版。2)对哺乳期小鼠乳房进行品红鉴定。尽管进行了强制早期断奶的试验，但在小鼠哺乳的乳房中没有观察到洋红色的现象，而是很容易看到细胞凋亡。3)为了将PAS阳性与洋红色核中单链DNA的积聚联系起来，对人乳房进行了多聚ADP-核糖免疫组织化学染色。据称，核蛋白的多聚ADP核糖基化被认为是针对DNA损伤的紧急宿主反应。洋红色核呈多聚ADP-核糖免疫反应，其邻近的二元醇成分可能解释了洋红色核的PAS反应。

Magentosis is a new mode of cell death that we found in a regressing stage of human lactating breast, histochemically featured by accumulation of single-stranded DNA in the PAS-positive (magenta-colored) nuclei. To clarify the biologic significance of magentosis, the following experiments were performed. 1) Varied human hormone-responsive organs were microscopically screened for this unique phenomenon. Except for a thyroid papillary carcinoma, magentotic nuclei were not demonstrated. Magentosis in thyroid cancer will be published soon. 2) We tried to identify magentosis in mouse lactating breast. In spite of the trials for compulsory early weaning, the magentotic phenmenon was not observed in the mouse lactating breast, where apoptosis was easily seen instead. 3) In order to correlate PAS positivity with the accumulation of single-stranded DNA in the magentotic nuclei, imunostaining for poly(ADP-ribose) was performed in the human breast. Allegedly, polyADP ribosylation of the nuclear proteins is regarded as an emergency host reaction against DNA damage. The magentotic nuclei were immunoreactive for poly(ADP-ribose), whose vicinal diol elements may explain the PAS reactivity in the magentotic nuclei.