The significance of polyADP ribosylation in 'magentosis', a new type of cell death: Molecular pathologic approaches to magentosis in a regressing stage of human lactating breast and other hormone-responsive organs

聚ADP核糖基化在“mageosis”（一种新型细胞死亡）中的意义：人类哺乳期乳房和其他激素反应器官退化阶段中magentosis的分子病理学方法

• 批准号: 10670176
• 负责人: TSUTSUMI Yutaka
• 金额: $ 2.3万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670176/
• 关键词: lactating breast; magntosis; cell death; single-stranded DNA; mouse model; poly (ADP-ribose); PAS staining; thyroid papillary carcinoma; 離乳; 実験モデル(ラット); ポリADPリボース; アポトーシス

Magentosis is a new mode of cell death that we found in a regressing stage of human lactating breast, histochemically featured by accumulation of single-stranded DNA in the PAS-positive (magenta-colored) nuclei. To clarify the biologic significance of magentosis, the following experiments were performed. 1) Varied human hormone-responsive organs were microscopically screened for this unique phenomenon. Except for a thyroid papillary carcinoma, magentotic nuclei were not demonstrated. Magentosis in thyroid cancer will be published soon. 2) We tried to identify magentosis in mouse lactating breast. In spite of the trials for compulsory early weaning, the magentotic phenmenon was not observed in the mouse lactating breast, where apoptosis was easily seen instead. 3) In order to correlate PAS positivity with the accumulation of single-stranded DNA in the magentotic nuclei, imunostaining for poly(ADP-ribose) was performed in the human breast. Allegedly, polyADP ribosylation of the nuclear proteins is regarded as an emergency host reaction against DNA damage. The magentotic nuclei were immunoreactive for poly(ADP-ribose), whose vicinal diol elements may explain the PAS reactivity in the magentotic nuclei.

洋红病是一种新的细胞死亡模式，我们发现在一个退化阶段的人类哺乳期乳腺癌，组织化学特征是单链DNA的积累PAS阳性（洋红色）的细胞核。为了阐明品红的生物学意义，进行了以下实验。1)对不同的人类免疫反应器官进行显微镜筛查，以发现这种独特的现象。除甲状腺乳头状癌外，未发现有核变。甲状腺癌中的品红现象即将发表。2)我们试图确定在小鼠泌乳乳房的品红。尽管进行了强制性早期断奶的试验，但在小鼠泌乳乳房中未观察到细胞变红现象，反而容易观察到细胞凋亡。3)为了将PAS阳性与核仁中单链DNA的积累相关联，在人乳腺中进行了聚（ADP-核糖）的免疫染色。此外，核蛋白的聚ADP核糖基化被认为是宿主对DNA损伤的紧急反应。胞核呈多聚腺苷二磷酸核糖免疫反应阳性，其邻位二醇成分可解释胞核PAS反应。

项目成果

Umemura S, et al: "Enhancement of TαT-mediated αUTP-biotin nick end-labeling(TUNNEL) method using mung bean nuclease; single-stranded DNA digestion enzyme"J. Histochem. Cytochem.. 44. 125-132 (1996)

Umemura S 等人：“使用绿豆核酸酶增强 TαT 介导的 αUTP-生物素缺口末端标记 (TUNNEL) 方法；单链 DNA 消化酶”J. Histochem. 44. 125-132 (1996)

Tsutsumi Y.: "cell proliferation and apoptosis. Stable cell, stabilized cell, and renewing cell"Igaku-no-Ayumi. 187(in Japanese). 463-464 (1998)

Tsutsumi Y.：“细胞增殖和凋亡。稳定细胞，稳定细胞和更新细胞”Igaku-no-Ayumi。

Umemura S, et al: "'Magentosis' in human lackting breast : A mode of cell death accumulehing single-stranded DNA stretches or breeks"Pathol. Int.. 46. 122-129 (1996)

Umemura S 等人：“缺乏乳房的人类‘Magentosis’：一种累积单链 DNA 延伸或断裂的细胞死亡模式”Pathol。

Umemura S.: "Cell death in human lactating breast"Igaku-no-Ayumi. 177(in Japanese). 298-299 (1996)

Umemura S.：“人类哺乳期乳房的细胞死亡”Igaku-no-Ayumi。

Umemura S, et al: "'Magentosis' in human lactating breast: A mode of cell death accumulating single-stranded DNA stretches or breaks"Pathol. Int.. 46. 122-129 (1996)

Umemura S 等人：“人类哺乳期乳房中的‘Magentosis’：一种累积单链 DNA 拉伸或断裂的细胞死亡模式”Pathol。