Expression of human endogenous retrovirus gene in tumor cells

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Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.