Expression of human endogenous retrovirus gene in tumor cells

人内源性逆转录病毒基因整合在染色体DNA中，通过顺序转录因子表达。我们研究了人内源性逆转录病毒基因克隆4-1启动子活性的LTR测定。利用荧光素酶法作为报告基因和Jarkat细胞对克隆4-1的LTR启动子片段进行了测定。我们确定184-bp片段具有启动子活性，242-bp片段对表达具有抑制活性。我们通过凝胶移位实验和体内脚印实验观察到184-bp片段存在DNA结合蛋白。我们的研究表明，人内源性逆转录病毒基因的表达是由ltr中的一个转录元件决定的，少量的DNA结合蛋白作为转录因子参与了这一过程。

Human endogenous retrovirus genes are integrated in the chromosomal DNA and expressed by ordinal transcription factors.We investigated the promoter activity determined by LTR in human endogenous retrovirus gene Clone 4-1.By using luciferase assay as reporter gene and Jarkat cell we determined promoter fragment in the LTR of Clone 4-1.We determined the 184-bp fragment having promoter activity and 242-bp fragment having suppressive activity on the expression.We observed the presence of DNA binding protein to the 184-bp fragment by gel shift assay and in vivo foot printing assay.Our study suggested that the expression of human endogenous retrovirus gene is determined by a transcriptional element in LTR.Small numbers of DNA binding protein as transcriptional factors are involved in this process.