Moluecular analysis of the lesion induced by SMP30

为了阐明这些酶在老年性疾病中的作用，研究了这些酶的活性。SMP30在肝脏中以有机磷酸的形式发挥作用。SMP30的DFPase活性需要镁、锰、钴等二价离子，而不需要钙。其真正的酶活性似乎是酸性磷酸酶。这一功能可能与溶酶体的功能有关。老年肾脏的形态特征为肾小管上皮细胞内有明显的脂褐素形成。这一发现表明，SMP30适合作为衰老标记。在糖尿病肾病中，SMP30在肾小管上皮细胞中的分布具有特征性。SMP30可用于病理诊断。Carolie限制延长了实验动物的寿命。随着寿命的延长，各脏器中SMP30的含量显着增加。

To elucidate of the roles in ageassociated diseases the enzyme activities were investigated. SMP30 works as organphosphatese in liver. DFPase activity of SMP30 requires divalent ions like as Mg, Mn, or Co but not Ca. The authentic enzyme activity is seemetd to be acid phosphatase. This function might be related with the function of lysosome. The morphological features of aged kidney showed marked formation of lipofuscin in renal tubular epithelia. This finding indicates that SMP30 is suitable for senescence marker. In diabetic nephropathy the distribution of SMP30 in tubular cells is characteristic. SMP30 will be useful for pathological diagnosis. Carolie restriction enhances the life span of experimental animals. Along with the elongated life-span the amounts of SMP30 in organs was significantly enhanced.