Moluecular analysis of the lesion induced by SMP30

为阐明其在相关疾病中的作用，对酶活性进行了研究。SMP30在肝脏中起有机磷酸酯的作用。SMP30的DFPase活性需要二价离子如Mg、Mn或Co，而不需要Ca。该酶的真正活性似乎是酸性磷酸酶。这种功能可能与溶酶体的功能有关。老年肾脏的形态学特征为肾小管上皮内明显形成脂褐素。这一发现表明SMP30适合作为衰老标志物。在糖尿病肾病中，SMP30在小管细胞中的分布具有特征性。SMP30将有助于病理诊断。卡洛里限制可以延长实验动物的寿命。随着寿命的延长，各器官中SMP30的含量显著增加。

To elucidate of the roles in ageassociated diseases the enzyme activities were investigated. SMP30 works as organphosphatese in liver. DFPase activity of SMP30 requires divalent ions like as Mg, Mn, or Co but not Ca. The authentic enzyme activity is seemetd to be acid phosphatase. This function might be related with the function of lysosome. The morphological features of aged kidney showed marked formation of lipofuscin in renal tubular epithelia. This finding indicates that SMP30 is suitable for senescence marker. In diabetic nephropathy the distribution of SMP30 in tubular cells is characteristic. SMP30 will be useful for pathological diagnosis. Carolie restriction enhances the life span of experimental animals. Along with the elongated life-span the amounts of SMP30 in organs was significantly enhanced.