The analysis of role of calcium-dependent protein kinase in the mechanism of parasitism of malarial parasites

钙依赖性蛋白激酶在疟原虫寄生机制中的作用分析

基本信息

  • 批准号:
    06670256
  • 负责人:
  • 金额:
    $ 1.34万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

In 1994 : Polymerase chain reaction (PCR) was carried out against P.yoelii genomic DNA with oligonucleotide primers which were designed from highly conserved regions among calcium-dependent protein kinases (protein kinase C and Ca^<2+>/calmodulin-dependent protein kinase) of a wide range of organisms and a PCR product was yielded. P.yoelii genomic DNA library was screened using this PCR product as a primer. Sequencing of the insert of a positive clone revealed that it contained a gene which codes a protein kinase.In 1995 : The comparison of the primary structure of the parasite protein kinase with those of various kinds of protein kinases of other organisms showed that the parasite protein belongs to cAMP-dependent protein kinase catalytic subunit family members. In situ gel assay of P.yoelii proteins showed that there are at least 8 kinds of protein kinases of P.yoelii. And the calcium-dependent protein kinase showed the highest activity from late trophozoite stage to schizont stage. The protein kinase which is more activated in the presence of EGTA showed the highest activity from schizont stage to early trophozoite stage. The study of the effect of various inhibitors of protein kinases on the growth of P.falciparum in vitro demonstrated that inhibitors of calcium-dependent protein kinases (protein kinase C and Ca^<2+>/calmodulin-dependent protein kinase) inhibit the growth of P.falciparum.
1994年:用寡核苷酸引物对约氏疟原虫基因组DNA进行聚合酶链反应(PCR),并产生PCR产物,所述寡核苷酸引物是从多种生物体的钙依赖性蛋白激酶(蛋白激酶C和Ca^2+/钙调蛋白依赖性蛋白激酶)中的高度保守区域设计的。用此PCR产物作为引物筛选约氏疟原虫基因组DNA文库。1995年:通过对寄生虫蛋白激酶的一级结构与其它生物体的各种蛋白激酶的一级结构的比较,表明寄生虫蛋白属于cAMP依赖的蛋白激酶催化亚基家族成员。约氏疟原虫蛋白质的原位凝胶分析表明,约氏疟原虫至少有8种蛋白激酶。钙依赖性蛋白激酶活性在滋养体晚期至滋养体期最高。在EGTA存在下更被激活的蛋白激酶显示出最高的活性从殖子阶段到早期滋养体阶段。体外研究了各种蛋白激酶抑制剂对恶性疟原虫生长的影响,结果表明钙依赖性蛋白激酶(蛋白激酶C和Ca^2+/钙调蛋白依赖性蛋白激酶)抑制剂抑制恶性疟原虫的生长。

项目成果

期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Atsuko Saito-Ito, Shenyi He, Masatsugu Kimura, Takeo Matsumura, Kazuyuki Tanabe.: "Cloning and structual analysis of the gene for cAMP-dependent protein kinase catalytic subunit from Plasmodium yoelii" Biochimica et Biophysica Acta.1269. 1-5 (1995)
Atsuko Saito-Ito、Shenyi He、Masatsugu Kimura、Takeo Matsumura、Kazuyuki Tanabe.:“约氏疟原虫 cAMP 依赖性蛋白激酶催化亚基基因的克隆和结构分析”Biochimica et Biophysicala Acta.1269。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Atsuko Saito-Ito: "Cloning and structual analysis of the gene for cAMP-dependent protein kinase catalytic subunit from Plasmodium yoelii" Biochimica et Biophysica Acta. 1269. 1-5 (1995)
Atsuko Saito-Ito:“来自约氏疟原虫的 cAMP 依赖性蛋白激酶催化亚基基因的克隆和结构分析”生物化学和生物物理学学报。
  • DOI:
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    0
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SAITO Atsuko其他文献

SAITO Atsuko的其他文献

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{{ truncateString('SAITO Atsuko', 18)}}的其他基金

Response to infant stimuli and the effects of stress on it: Using attention task
对婴儿刺激的反应以及压力对其的影响:使用注意任务
  • 批准号:
    23700307
  • 财政年份:
    2011
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Social learning and endocrinology in primate infants
灵长类婴儿的社会学习和内分泌学
  • 批准号:
    21700284
  • 财政年份:
    2009
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
A study of the phenomenon of high concentration and high speed degradation of polycyclic aromatic hydrocarbons mediated through annelids.
环节动物介导的多环芳烃高浓度高速降解现象的研究。
  • 批准号:
    21710015
  • 财政年份:
    2009
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Research of antigenic diversity and phylogenetic marker genes for Babesia microti
田鼠巴贝虫抗原多样性及系统发育标记基因研究
  • 批准号:
    15590367
  • 财政年份:
    2003
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of new malaria parasite detection system : Establishment of automatic and simple system
新型疟疾寄生虫检测系统的开发:建立自动化且简易的系统
  • 批准号:
    10557030
  • 财政年份:
    1998
  • 资助金额:
    $ 1.34万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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