Research of antigenic diversity and phylogenetic marker genes for Babesia microti

田鼠巴贝虫抗原多样性及系统发育标记基因研究

基本信息

  • 批准号:
    15590367
  • 负责人:
  • 金额:
    $ 2.3万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

In this study, we newly identified or established several isolates or strains of quasi-Otsu-SSUrDNA-type (Nagano SSUrDNA-type) and US-SSUrDNA-type Babesia microti in addition to Kobe-SSUrDNA-type and Otsu-SSUrDNA-type of B. microti from Japanese rodents. Nagano-SSUrDNA-type B. microti was exclusively identified in Eothenomys andersoni, while Otsu-SSUrDNA-type B. microti was identified in Apodemus species in Nagano, where quasi-Otsu-and Otsu-SSUrDNA-type B. microti are endemic. We also identified or established isolates or strains of Kobe-SSUrDNA-type B. microti (the Meishan isolate) from Taiwanese rodents.For these isolates or strains plus the Gl, the N/A, the Kobe, the Otsu and the Hachimaki strains, which had been previously established, the sequences of ITS1/ITS2 were investigated. The percent identities of ITS1/ITS2 between the different SSUrDNA-types were around 50-60%. Within the Otsu-SSUrDNA-type, the ITS1 and ITS2 sequences of all B. microti isolates were different only by 1-3 … More nucleotides from each other. For the Kobe-SSUrDNA-type of B. microti, all of the isolates from Japanese rodents possessed completely identical ITS1/1TS2 sequences. However, between the Kobe and Meishan strains, around 92% identities were showed. For the US-SSUrDNA-type of B.microti, the ITS sequences of the GI and N/A strains were completely identical, while the ITS1/ITS2 sequence of the Japanese isolate was clearly differentiated from those of the GI and N/A strains with the 91.1 %-97.2% identities.The main immunoreactive antigens of the Kobe and Otsu strains were examined by immunoblot analysis and were shown to be approximately 42kD and 35kD, respectively. Between the same SSUrDNA-type B. microti with different ITS1/ITS2 sequences, the molecular weights of main immunoreactive antigens seemed to be somewhat different, although indirect fluorescent antibody test showed cross immunoreactivities between the same SSUrDNA-type B. microti with different ITS1/ITS2 sequences. Because we could not clone the main immunoreactive antigens, more detailed structural analysis for immunoreactive antigens of the isolates or strains was impossible. Less
在本研究中,除了B的Kobe-SSUrDNA-型和Otsu-SSUrDNA-型之外,我们新鉴定或建立了几个准大津-SSUrDNA-型(长野SSUrDNA-型)和US-SSUrDNA-型的东方Babelicroti分离物或菌株。来自日本啮齿动物的田鼠。长野SSUrDNA B型。microti仅在Eothenomys andersoni中鉴定,而Otsu-SSUrDNA为B型。在长野的姬鼠种中鉴定出microti,其中准大津型和大津型SSUrDNA B型。田鼠是地方性的。我们还鉴定或建立了Kobe-SSUrDNA-B型的分离株或菌株。对这些分离株和已建立的G1、N/A、科比、大津和八卷株进行ITS 1/ITS 2序列分析。不同SSUrDNA类型之间ITS 1/ITS 2的百分比相同性在50- 60%左右。在Otsu-SSUrDNA型中,所有B. microti菌株之间的差异仅为1-3 ...更多信息 彼此的核苷酸。对于Kobe-SSUrDNA-类型的B。所有日本啮齿动物分离株的ITS 1/1 TS 2序列完全相同。而科比株与梅山株的同源性在92%左右。对于US-SSUrDNA型的B.microti,GI和N/A菌株的ITS序列完全相同,日本分离株ITS 1/ITS 2序列与GI和N/A分离株的ITS 1/ITS 2序列有明显的差异,差异率为91.1%~ 97.2%。通过免疫印迹分析检查科比和大津菌株的主要免疫反应性抗原,并且显示为约42 kD,35kD。相同SSUrDNA类型B之间。不同ITS 1/ITS 2序列的田鼠,其主要免疫反应抗原的分子量似乎有所不同,但间接荧光抗体试验显示同一SSUrDNA B型之间存在交叉免疫反应。microti的ITS 1/ITS 2序列不同。由于我们不能克隆主要的免疫反应性抗原,因此不可能对分离株或菌株的免疫反应性抗原进行更详细的结构分析。少

项目成果

期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Identification of a new type of Babesia species in wild rats (Bandicota indica) in Chaing Mai Province, Thailand
泰国清迈省野鼠(Bandicota indica)中新型巴贝虫的鉴定
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Dantrakool;Anchalee;et al.
  • 通讯作者:
    et al.
Babesiosis
  • DOI:
    10.1002/0471743984.vse0844
  • 发表时间:
    2012-06
  • 期刊:
  • 影响因子:
    2.6
  • 作者:
    Melissa Butler-Haughton
  • 通讯作者:
    Melissa Butler-Haughton
Dantrakool, A., Somboon, P., Hashimoto, T., Saito-Ito, A.: "Identification of a new type of Babesia species in wild rats (Bandicota indica) in Chiang Mai Province, Thailand"Journal of Clinical Microbiology. 42・2. 850-874 (2004)
Dantrakool, A.、Somboon, P.、Hashimoto, T.、Saito-Ito, A.:“泰国清迈省野生大鼠(Bandicota indica)中新型巴贝虫的鉴定”《临床微生物学杂志》。 42・2。850-874(2004)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
斎藤あつ子: "バベシア症"獣医寄生虫学会誌. 2・1. 21-26 (2003)
齐藤敦子:“巴贝斯虫病”兽医寄生虫学会杂志 2・1(2003 年)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Identification of a new type of Babesia species in wild rats (Bandicota indica) in Chiang Mai Province, Thailand
  • DOI:
    10.1128/jcm.42.2.850-854.2004
  • 发表时间:
    2004-02-01
  • 期刊:
  • 影响因子:
    9.4
  • 作者:
    Dantrakool, A;Somboon, P;Saito-Ito, A
  • 通讯作者:
    Saito-Ito, A
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SAITO Atsuko其他文献

SAITO Atsuko的其他文献

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{{ truncateString('SAITO Atsuko', 18)}}的其他基金

Response to infant stimuli and the effects of stress on it: Using attention task
对婴儿刺激的反应以及压力对其的影响:使用注意任务
  • 批准号:
    23700307
  • 财政年份:
    2011
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Social learning and endocrinology in primate infants
灵长类婴儿的社会学习和内分泌学
  • 批准号:
    21700284
  • 财政年份:
    2009
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
A study of the phenomenon of high concentration and high speed degradation of polycyclic aromatic hydrocarbons mediated through annelids.
环节动物介导的多环芳烃高浓度高速降解现象的研究。
  • 批准号:
    21710015
  • 财政年份:
    2009
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of new malaria parasite detection system : Establishment of automatic and simple system
新型疟疾寄生虫检测系统的开发:建立自动化且简易的系统
  • 批准号:
    10557030
  • 财政年份:
    1998
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The analysis of role of calcium-dependent protein kinase in the mechanism of parasitism of malarial parasites
钙依赖性蛋白激酶在疟原虫寄生机制中的作用分析
  • 批准号:
    06670256
  • 财政年份:
    1994
  • 资助金额:
    $ 2.3万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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