Ultrastructural Analysis of Pore-Formation by Streptolysin 0

采用阴性染色、免疫电镜、电子能谱成像等方法对溶血素0 （SLO）在红细胞膜上的成孔机制进行了超微结构分析。将SLO作用于红细胞膜，在0‰温度下作用3 ~ 10 min，未检测到环状结构。但在室温下，将样品分别用抗slo和胶体金共轭抗igg处理每20分钟，观察与膜结合的金颗粒。这些结果表明，第一步，SLO分子与红细胞膜的结合是温度无关的。将培养温度升至37ºC，培养3 min后，膜上出现一个带慢环的孔。这一结果表明，第二步——分子聚集在一起，形成带孔的环——与温度有关。此外，还证实了SLO在脂质体上形成的环状结构也是由内、外两层带冠的双层结构组成。

The mechanism of pore formation by streptolysin 0 (SLO) on the erythrocyte membrane was ultrastrucurally analyzed by the methods of negatively stained, immuno-electron microscopy, and electron spectroscopic imaging.When SLO was applied to the erythrocyte membrane at 0ﾟC for 3 to 10 min, no ring-shaped structure was detected. But in the case of the specimen was then treated with anti-SLO and colloidal gold-conjugated anti-IgG for each 20 min at room temperature, the gold particles bound to the membrane were observed. These result indicated that the first step, the binding of SLO molecules to the erythrocyte-membrane was temperature independent.A hole with SLO-ring was found on the membrane after rising the incuvation temperature up to 37ﾟC for 3 min. This result suggests that the second step that the molecules meet together, and form the rings with holes was temperature dependent.Furthermore, it was confirmed that the ring structure forming on the liposome by SLO also composed of a double layr of an inner and an outer with a crown.