Recognition mechanism of the RNA segments of influenza virus by the NS2 protein at the virion assembly step.

NS2 蛋白在病毒粒子组装步骤中识别流感病毒 RNA 片段的机制。

• 批准号: 06670335
• 负责人: ODAGIRI Takato
• 金额: $ 1.41万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670335/
• 关键词: influenza virus; DI virus; DI-RNA; virion assembly; packaging of RNA; RNA transfection; NS2 protein; NS2蛋白; RNA転写・複製; ゲノムRNAパッケージング; 干渉

In the present research using NS2 mutant of influenza virus that produces a large amount of DI particles, we have studied (1) whether genomic RNA packaging into virion is suppressed by DI-RNA species, (2) whether there is a signal sequence which can recognize each RNA segment ; and (3) what is the function of NS2 protein during assembly of genome RNA segments.We found (1) that PA DI-RNA species selectively suppressed the incorporation of only the progenitor PA gene, (2) that 7 and 3 nucleotides at the 3' and 5' terminal ends, respectively, in the segment-specific noncoding regions functioned as the recognition signals of each RNA segment, and (3) that NS2 protein down-regulated the DI-RNA packaging, resulting in preferential incorporation of normal genomic RNAs.

在本研究中，我们使用产生大量DI颗粒的流感病毒的NS 2突变体，研究了（1）基因组RNA包装入病毒体是否被DI-RNA种类抑制，（2）是否存在能够识别每个RNA片段的信号序列;（3）NS 2蛋白在基因组RNA片段组装过程中的作用，我们发现（1）PA DI-RNA种类选择性地抑制仅祖PA基因的掺入，（2）片段特异性非编码区3'和5'末端分别有7个和3个核苷酸作为每个RNA片段的识别信号;（3）NS 2蛋白下调DI-RNA包装，导致正常基因组RNA优先掺入。

项目成果

T.Odagiri: "Reversed misreading of a GC doublet by the modified T7" Virus Genes. 8. 271-274 (1994)

T.Odagiri：“通过修饰的 T7 逆转了 GC 双联体的误读”病毒基因。

T.Odagiri: "An amino acid change in the nonstructural NS2 protein of an" J.Gen.Virol.75. 43-53 (1994)

T.Odagiri：“J.Gen.Virol.75 的非结构 NS2 蛋白中的氨基酸变化。”

T.Odagiri: "The role of the NS2 protein of influenza virus during the production of DI virus particles." Tohoku Colony. 29. 27-38 (1994)

T.Odagiri：“流感病毒 NS2 蛋白在 DI 病毒颗粒产生过程中的作用。”

T. Odagiri: "Reversd misreading of a GC dublet by the modified T7 ・・・" virus Genes. 8. 271-274 (1994)

T. Odagiri：“修改后的 T7 对 GC 双联体的逆转误读……”病毒基因。 8. 271-274 (1994)

T.Odagiri: "An amino acid change in the nonstructural NS2 protein" J.Gen.Virol.75. 43-53 (1994)

T.Odagiri：“非结构 NS2 蛋白中的氨基酸变化”J.Gen.Virol.75。