Study on the function of NS2 protein of influenza A virus at the assembly step of the genomic RNA segments.

甲型流感病毒NS2蛋白在基因组RNA片段组装步骤中的功能研究。

• 批准号: 09670318
• 负责人: ODAGIRI Takato
• 金额: $ 2.05万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670318/
• 关键词: influenza virus; CAT assay; influenza virus DI RNA; genomic RNA segments; discrimination of RNA segments; ゲノムRNA構造

The genome of influenza A virus consists of eight RNA segments of negative polarity. A set of eight RNA segments is required for an influenza virus particle to express infectivity. However, the mechanism of assembly or packaging of the RNA segments into an infectious particle remains unknown. In the present study using the reverse genetic system, we demonstrated that each RNA segment contained the recognition signal sequences for discriminate each other in the step of virion assembly.Moreover, by the gene expression system in which the recombinant influenza virus like genome RNA was cotransfected with the defective interfering (Dl) RNA derived from the PA gene or the PB2 gene, we identified that the nucleotide residues from 13 to 19 in the 3' terminal region of genome RNA segment were involved in the segment recognition signal.

甲型流感病毒基因组由8个负极性的RNA片段组成。流感病毒颗粒需要一组八个RNA片段才能表达传染性。然而，将RNA片段组装或包装成具有感染性的颗粒的机制仍不清楚。在利用反向遗传系统的研究中，我们证明了在病毒粒子组装的过程中，每个RNA片段都含有相互识别的识别信号序列，并且通过重组流感病毒类基因组RNA与来自PA基因或PB2基因的缺陷型干扰(DL)RNA共转染的基因表达系统，我们鉴定了基因组RNA片段3‘端13~19个核苷酸残基参与了片段识别信号。

项目成果

小田切 孝人: "インフルエンザウイルスゲノムRNA分節特異的非コード領域の機能" 日本臨床. 55,No10. 2547-2554 (1997)

Takato Odagiri：“流感病毒基因组RNA片段特异性非编码区的功能”Nippon Clinical，55，No.10。2547-2554（1997）

Takata,H.et al.: "L^* protein of the DA strain of Theiler's murine encephalomyelitis" Journal of Virology. 72. 4950-4955 (1998)

Takata,H.et al.：“泰勒氏鼠脑脊髓炎 DA 株的 L^* 蛋白”病毒学杂志。

小田切孝人: "インフルエンザのウイルス学" 臨床と微生物. 25. 645-653 (1998)

Takato Odagiri：“流感病毒学”临床和微生物学 25. 645-653 (1998)。

小田切 孝人: "インフルエンザウイルスの生態" Infection Control. 7. 16-21 (1998)

Takato Odagiri：“流感病毒的生态学”感染控制。7. 16-21 (1998)。

小田切孝人: "インフルエンザウイルスの生態" Infection Control. 7. 1398-1403 (1998)

Takato Odagiri：“流感病毒的生态学”感染控制。7. 1398-1403 (1998)。