RAPID TEST FOR DETECTION,TYPING AND SUBTYPING INFLUENZA VIRUSES IN CLINICAL SPECIMENS

临床标本中流感病毒的快速检测、分型和亚型分析

• 批准号: 06670443
• 负责人: MAEDA Akiko
• 金额: $ 1.15万
• 依托单位: OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670443/
• 关键词: VIRUS; INFRUENZA; MONOCLONAL-ANTIBODIES; IDENTIFICATION; DIAGNOSIS; インフルエンザウイルス; 酵素抗体法; PAP染色; 診断法; 迅速診断; -

A rapid culture assay whitch allows for the simultaneous typing and subtyping of currently circulating influenza A (H1N1), A (H3N2) and B viruses in clinical specimens was developed, monoclonal antibodies against influenza A (H1N1) : C179 and A(H3N2) : F49 and polyclonal antibody against influenza B,were used for immunoperoxidase staining of antigens infected MDCK cells. Eecept 2 strains, monoclonal antibodies and polyclonal antibody react exclusively with influenza A viruses and influenza B Virus, as determined with 160 influenza virus reference strains typed or subtyped by the hemagglutination-inhibition test. 2 strains were proved the mixed infection of influenza AH1 and AH3 by immunoperoxidase staining of antigens infected MDCK cells. To determine if the technique can be used as a rapid diagnostic test, on 1993/94 and 1994/95 epidemics of influenza, 72 and 80 clinical specimens were inoculated into MDCK cells. After 40 hours incubation, the cells were fixed and viral antigens were detected by immunoperoxidase staining. Influenza A viruses of the H1 and H3 subtypes were detected in 27 (1994/95) and 25(1993/94) specimems, respectively. The subtypes of 8 influenza AH3 and 7 influenza AH1 virus-positive specimems could not be determind because they conteined too little virus. We conclude that this assay is a rapid and relatively inexpensive test for detection, typing and subtyping influenza viruses in clinical specimens.

快速培养分析允许开发临床标本中的A（H3N2）和B病毒的当前循环流感A（H1N2）和B病毒的同时键入和亚型。抗原感染的MDCK细胞的免疫过氧化物酶染色。 EACEPT 2菌株，单克隆抗体和多克隆抗体仅与流感A病毒和流感B病毒反应，并由160个流感病毒参考菌株确定或通过血凝集抑制测试键入或亚型。 2菌株被抗原感染的MDCK细胞的免疫过氧化物酶染色证明了流感AH1和AH3的混合感染。为了确定该技术是否可以用作快速诊断测试，在1993/94和1994/95流感，72和80个临床标本的流行病中被接种到MDCK细胞中。孵育40小时后，固定细胞并通过免疫过氧化物酶染色检测病毒抗原。在27（1994/95）和25（1993/94）标本中检测到H1和H3亚型的流感病毒。无法确定的8种流感AH3和7种流感AH1病毒阳性标本的亚型，因为它们扭曲了太少的病毒。我们得出的结论是，该测定是在临床标本中进行检测，打字和亚型流感病毒的快速且相对便宜的测试。

项目成果

奥野 良信: "モノクロナール抗体を用いたインフルエンザの迅速診断" 治療. 77. 1220-1221 (1995)

Yoshinobu Okuno：“使用单克隆抗体快速诊断流感”治疗。77. 1220-1221 (1995)。

前田 章子、加瀬 哲男、奥野 良信: "モノクロナール抗体を利用したインフルエンザの迅速診断" INNERVISION(インナービジョン). (発表予定). (1995)

Akiko Maeda、Tetsuo Kase、Yoshinobu Okuno：“使用单克隆抗体快速诊断流感”INNERVISION（计划演示）。

Akiko MAEDA,Tetsuo KASE,Yoshinobu OKUNO: "RAPID TEST FOR DETECTION,TYPING AND SUBTYPING INFLUENZA VIRUSES IN CLINICAL SPECIMEMS" INNERVISION. in press. (1996)

Akiko Maeda、Tetsuo KASE、Yoshinobu OKUNO：“临床标本中流感病毒的快速检测、分型和分型”INNERVISION。

Yoshinovu OKUNO: "RAPID TEST FOR DETECTION INFLUENZA VIRUSES" J.Therap.77. 1220-1221 (1995)

Yoshinovu OKUNO：“检测流感病毒的快速测试”J.Therap.77。

奥野良信: "モノクロナール抗体を用いたインフルエンザの迅速診断法" 治療. 77. 1220-1221 (1995)

Yoshinobu Okuno：“使用单克隆抗体的流感快速诊断方法” 77. 1220-1221 (1995)。