Autoantigen and T cell receptor on infiltrating T cell in labial salivary glands from patients with Sjogren's syndrome.

我们分析了干燥综合征（SS）患者唇唾液腺T细胞上的T细胞受体（TCR），并澄清了以下结果。1) SS患者唇唾液腺浸润T细胞TCR vβ的表达不受限制；然而，Vbeta2-和Vbeta13阳性T细胞在唇部特异性和优先扩增（j . cline . invest .）。89: 681,1992)。2) ss患者浸润T细胞的TCR瓣膜库相当有限，jα基因的使用相对有限，提示自身反应性T细胞识别的抗原表位较少(J.Rheumatol.；[21][1655] 1994。3)部分浸润的Vbeta2-和Vbeta13-阳性T细胞克隆扩增，表明MHC上的抗原识别是抗原驱动的刺激。jβ基因的使用受到限制，提示超抗原不能刺激嘴唇中扩增的T细胞(关节炎，35:1362,1992;Br.J.Rheum。33: 420,1994)。此外，我们获得的证据表明，在SS患者的唇唾液腺中检测到Ro/SS- a 52kD反应性T细胞系的频率很高（25%），这表明Ro/SS- a 52kD可能是一种被自身反应性T细胞识别的自身抗原。Ro/SS-A 52kD蛋白上的T细胞表位分析表明，在Ro/SS-A 52kD的AA190-198和AA197-245上至少存在两个不同的T细胞表位。22: 2092,1995)。这些发现为SS提供了一种新的特异性治疗方法，即通过接种类似肽或抗tcr抗体选择性地使自身反应性T细胞失活。

We analyzed T cell receptor (TCR) on T cells in labial salivary glands from patients with Sjogren's syndrome (SS) and clarified the following results.1) The repertoire of TCR Vbeta of infiltrating T cells in the labial salivary glands of patients with SS was not restricted ; however, the Vbeta2- and Vbeta13 positive T cells expanded specifically and preferentially in the lips (J.Clin.Invest.89 : 681,1992).2) TCR Valpha repertoire of infiltrating T cells is rather restricted in the individual patient with SS.The usage of Jalpha gene was relatively limited, suggesting a few epitopes of antigen recognized by autoreactive T cells (J.Rheumatol.21 : 1655,1994).3) Some infiltrating Vbeta2- and Vbeta13- positive T cells were clonally expanded, indicating the antigen-driven stimulation through the recognition of antigen on MHC.The usage of Jbeta gene was restricted, suggesting that expanded T cells in the lips are not stimulated by superantigens (Arthritis Rheum.35 : 1362,1992 ; Br.J.Rheum.33 : 420,1994).Moreover, we obtained evidence that Ro/SS-A 52kD reactive T cell lines were detected in labial salivary glands from SS patients at high frequency (25%), suggeting that Ro/SS-A 52kD is a possible autoantigen recognized by autoreactive T cells. Analysis of T cell epitopes on Ro/SS-A 52kD protein showed that there exist at least two different T cell epitopes on AA190-198 and AA197-245 of Ro/SS-A 52kD (J.Rheumatol.22 : 2092,1995).These findings give to a new specific therapeutic approach to SS via the selective inactivation of auto-reactive T cells by vaccination with analog peptides or anti-TCR antibodies.