EFFECT OF HEAT TREATMENT OF TUMOR CELL ON LYMPHOKINE ACTIVATED KILLER CELL CYTOTOXICITY

点击翻译按钮获取中文摘要

The aim of this study is to demonstrate the effect of heat treatment of tumor cells on the cytotoxicity of these cells by lymphokine activated killer cell (LAK cell). Human hepatocellular carcinoma cell line, Huh-7 was used as target cell, and to evaluate both the cell proliferation of tumor cells and LAK activity, a new assay system was established using Alamar blue. At first, tumor cells were incubated at 41.0ﾟC,43.0ﾟC or 45.0ﾟC,then the proliferation rates of these treated cells were measured. Secondly, LAK cells and/or tumor cells were heated before LAK assay and the heat effect of these cells on LAK activity were evaluated. Finally, LAK assays were performed before or after heat treatment, then the total cytotoxicity induced by LAK cells and heat treatment were compared.Results :1. Tumor cells heated at 41.0ﾟC for 1-6 hours or at 43.0ﾟC or 45.0ﾟC for 1 hour showed the same proliferative activities as that of tumor cells without heat. On the other hand, heat treatment at 43.0ﾟC or 45.0ﾟC for 3-6 hours completely inhibited tumor growth.2. Heat treatment of tumor cells enhanced LAK activity by non-treated LAK cells.However, heat-treated LAK cells failed to show cytotoxicity against both heat-treated and not treated tumor cells.3. Heat treatment of tumor cells before LAK assay induced remarkable cytotoxic effect. However, when tumor cells were heated after LAK assay, the total cytotoxicity did not increase. Simultaneous LAK assay with heat showed little cytotoxicity against tumor cells.