IMMUNO-REGULATION OF AUTO-REACTIVELIVER-INFILTRATE LYMPHOCYTE AND ITS RELATION TO APOPTOSIS IN AUTOMMUNE HEPATITIS.

自身反应性肝浸润淋巴细胞的免疫调节及其与自身免疫性肝炎细胞凋亡的关系。

• 批准号: 06670585
• 负责人: ZENIYA Mikio
• 金额: $ 1.34万
• 依托单位: The Jikei University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670585/
• 关键词: autoimmune hepatitis; T-cell receptor; Fas; bcl-2; immune coplex; hepatitis C; 慢性肝炎; bc1; TCRVβ; bcl-2; TCRレパトア; FACS

The existence of T-cell receptor V beta chain clonarity was observed in liver-infiltrating lymphocyte of autoimmune hepatitis patients who were diagnosed as active stage clinically by sequence analysis using PCR method. This clonality was diminished when the patient became remission. Two-color flow-cytometry technique was established for detecting both Fas and bcl-2 of peripheral lymphocytes. In chronic liver disease patients, the percent of both CD4/Fas and CD8/Fas positive cell was increased compared to healthy controls. The correlation between these increases and clinical findings including serum immunological markers, such as autoantibody, immunoglobulin level, and liver function test was not observed. There was no differences in CD19/bcl positive cells between two groups. In chronic hepatitis C,serum level of C3d-binding immune complex was increased and this increase related to quaispiesies of hepatitis C virus confirmed by PCR-SSCP using primers encoded in hypervariable region of hepatitis C virus.

应用PCR方法对临床诊断为活动期的自身免疫性肝炎患者肝浸润淋巴细胞进行序列分析，观察到T细胞受体V β链克隆性的存在。当患者病情缓解时，这种克隆性减弱。建立了双色流式细胞术检测外周血淋巴细胞Fas和bcl-2的方法。与健康对照相比，慢性肝病患者中CD 4/Fas和CD 8/Fas阳性细胞的百分比均增加。未观察到这些增加与临床结果（包括血清免疫学标志物，如自身抗体、免疫球蛋白水平和肝功能检查）之间的相关性。CD 19/bcl阳性细胞在两组间无差异。在慢性丙型肝炎，血清C3 d结合免疫复合物的水平增加，这种增加有关的丙型肝炎病毒的quasipieses证实的PCR-SSCP使用的引物编码的丙型肝炎病毒的高变区。

项目成果

Zeniya M et al: "The present status of autoimmune hepatitis and its heterogeneity in Hepatology I" Excepta Medica.(Eds Yamanaka M et al), 101-118 (1995)

Zeniya M 等人：“自身免疫性肝炎的现状及其在肝病学中的异质性 I”Exceta Medica。（Eds Yamanaka M 等人），101-118（1995）

大川康彦他: "C型慢性肝炎におけるインターフェロン治療による血清U-lraおよびU-10の経時的変化" 消化器と免疫. 30. 155-159 (1995)

Yasuhiko Okawa 等人：“慢性丙型肝炎中干扰素治疗引起的血清 U-1ra 和 U-10 水平的时间变化”，《胃肠病学和免疫学》，30. 155-159 (1995)。

Mikio Zeniya, et al: "Elsevier" Genetic Background of Autoimmune hepatitis in Japan in Autoimmune hepatotis. Ed. Nishioka, Toda, Zeniya,267-280 (1994)

Mikio Zeniya 等人：“Elsevier”自身免疫性肝炎中日本自身免疫性肝炎的遗传背景。

Karasawa T et al: "Genetic hetetogeneity in the precore region of hepatitis B virus in hepatitis Be antigen-negative chronic hepatitis B patients." J Med Virol. 43. 373-380 (1994)

Karasawa T 等人：“Be 抗原阴性慢性乙型肝炎患者乙型肝炎病毒前核心区的遗传异质性。”

Mikio ZENIYA et al: "Thrombomodulin expression of sinusoidal endothelial cells in chronic viral hepatitis" J. Gastro. Hepat,. 10. s77-S80 (1995)

Mikio ZENIYA 等人：“慢性病毒性肝炎中窦内皮细胞的血栓调节蛋白表达”J. Gastro。