Protease as the pathogenic factor of Pseudomonas cepacia pneumonia

蛋白酶是洋葱假单胞菌肺炎的致病因子

• 批准号: 06670617
• 负责人: FUJITA Jiro
• 金额: $ 1.34万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670617/
• 关键词: Pseudomonas cepacia; pneumonia; protease; Pseudomenas cepacia

The present study was designed to examine the role of protease as a pathogenic determinant of P.cepacia. Clinically isolated strain of P.cepacia (KF-1), which was derived from a patient with P.cepacia pneumonia, and produced a significant amount of protease was used in this study. In comparison, the strain KFT1007, which was produced from KF-1 by the insertion of a single transposont gene, and lost the ability to release protease was also used. Immunosuppressed mice were challenged by intratracheal inoculation of 2*10^8 colony-forming units of P cepacia, and the survival rates were recorded. Between the strains which could produce protease and could not produce protease, there was an obvious difference in survival rates. In conclusion, our study suggests that protease plays an important role in the pathogenesis of P.cepacia.

本研究的目的是研究蛋白酶的作用，作为一个致病性决定因素的洋葱疫霉。在本研究中使用洋葱假单胞菌的临床分离菌株（KF-1），其来源于洋葱假单胞菌肺炎患者，并产生显著量的蛋白酶。作为比较，还使用了KFT 1007菌株，该菌株通过插入单个转座子基因由KF-1产生，并且失去了释放蛋白酶的能力。免疫抑制小鼠经阴道接种2 × 10^8集落形成单位的洋葱伯克霍尔德菌进行攻击，并记录存活率。产蛋白酶菌株与不产蛋白酶菌株的存活率存在明显差异。总之，我们的研究表明，蛋白酶起着重要的作用，洋葱疫霉的发病机制。

项目成果

藤田次郎: "ブドウ糖非発酵グラム陰性桿菌による院内感染肺炎について" 日本胸部臨床. 53. 327-331 (1994)

Jiro Fujita：“关于非葡萄糖发酵革兰氏阴性杆菌引起的医院获得性肺炎”日本胸科诊所。 53. 327-331 (1994)

J.Fujita, K.Negayama, J.Takahara: "Comparison of in vitro activity of carbapenems and tosufloxacin against clinically-isolated strains of Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis" Chemotherapy (Tokyo). 43. 187-192 (1995)

J.Fujita、K.Negayama、J.Takahara：“碳青霉烯类和托磺沙星对临床分离的铜绿假单胞菌、金黄色葡萄球菌和粪肠球菌菌株的体外活性比较”化疗（东京）。

Hiroshi Miyawaki: "Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction" Diag Microbiol Infect Dis. 23. 77-83 (1995)

Hiroshi Miyawaki：“通过任意引发的聚合酶链反应调查洋葱假单胞菌引起的院内呼吸道感染”Diag Microbiol Infect Dis。

Jiro Fujita: "comparison of in vitro activity of carbapenems and tosufloxacin against clinically-isolated strains of pseudomonas aeruginosa,Staphylococcus aureus and Enterococcus faecalis" Chemotherapy (Tokyo). 43. 187-192 (1995)

Jiro Fujita：“碳青霉烯类和托舒沙星对铜绿假单胞菌、金黄色葡萄球菌和粪肠球菌临床分离菌株的体外活性比较”化疗（东京）。

Keiichi Takigawa: "Comparing antimicrobial activity against resistant Pseudomonas aeruginosa using an index for the absence of cross-resistance" J Antimicrob Chemther. 35. 425-427 (1995)

Keiichi Takikawa：“使用不存在交叉耐药性的指数比较对耐药性铜绿假单胞菌的抗菌活性”J Antimicrob Chemther。