Establishment of typing system of MRSA by arbitrarily-primed PCR

任意引物PCR法MRSA分型体系的建立

• 批准号: 09670617
• 负责人: FUJITA Jiro
• 金额: $ 1.92万
• 依托单位: Kagawa Medical university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670617/
• 关键词: Arbitrarily-primed PCR; MRSA; Typing; Nosocomial infection

In 1997, we established the typing method for methicillin-resistant Staphylococcus aureus (MRSA) by an arbitrarily-primed polymerase. Chain reaction. In 1998, we applied this method to several strains of MRSA isolated in Kagawa Medical University Hospital. Usefulness of the typing method by AP-PCR was compared to other typing methods: phenotypic characteristics (production of enterotoxin and TSST-1, antimicrobial susceptibity) and molecular typing procedure (plasmid DNA profile, pulsed-field gel electrophoresis [PFGE] . Although traditional epidemiological methods for example, coagulase typing plays a central role in hospital infection control, combination of plasmid DNA profile, AP-PCR, and PFGE may prove to be a particularly informative means of tracking the nosocomial spread of MRSA. Using AP-PCR, we could prove the nosocomial infection caused by cagulase VII positive MRSA strain .In 1999, we applied this method to other bacteria : Pseudomonas cepacia, Pasteurella multocida, and Mycobacterium avium intracellulare complex, and we demonstrated that AP-PCR could be a useful and simple method to investigate the transmission of several bacteria.

1997年，我们建立了用任意引物聚合酶对耐甲氧西林金黄色葡萄球菌（MRSA）进行分型的方法。连锁反应。1998年，我们将该方法应用于香川医科大学医院分离的几株MRSA。将AP-PCR分型方法与其他分型方法的有效性进行比较：表型特征（肠毒素和TSST-1的产生，抗菌药物敏感性）和分子分型程序（质粒DNA谱，脉冲场凝胶电泳[PFGE]）。虽然凝血酶分型等传统流行病学方法在医院感染控制中发挥着核心作用，但质粒DNA谱、AP-PCR和PFGE的结合可能被证明是追踪MRSA医院传播的一种特别有用的方法。应用AP-PCR技术，我们可以证实由偶联酶VII阳性MRSA菌株引起的医院感染。1999年，我们将该方法应用于其他细菌：洋葱假单胞菌、多杀性巴氏杆菌和鸟分枝杆菌胞内复合体，我们证明了AP-PCR可以是一种有用且简单的方法来研究几种细菌的传播。

项目成果

Yuka Obayash: "Investigation of rnosocomial infection due to arbekacln-resistant methicillin-reslstant Staphylococcus aureus"Diag Microbiol Infect Dis. 28. 53-59 (1997)

Yuka Obayash：“耐阿贝卡林、耐甲氧西林金黄色葡萄球菌引起的医院感染的调查”Diag Microbiol Infect Dis。

Yuka Obayashi: "Clinical features of non-tuberculous mycobacterium.-Comparison between smear-positive cases and culture-positive cases,as well as comparison between Mycobacterium avium and Mycobacterium intracellulare-"Int J Tuberc Lung Dis. 2. 597-602 (1

Yuka Obayashi：“非结核分枝杆菌的临床特征。-涂片阳性病例与培养阳性病例的比较，以及鸟分枝杆菌与胞内分枝杆菌的比较-”Int J Tuberc Lung Dis。

Yuka Obayashi: "Clincal features of non-tuberculous mycobacterium.-Comparison between smear-positive cases and culture-positive cases,as well as comparison between Mycobacterium avium and mycobacterium intracellulare-" Int J Tuberc Lung Dis. 2. 597-602 (1

Yuka Obayashi：“非结核分枝杆菌的临床特征。-涂片阳性病例与培养阳性病例之间的比较，以及鸟分枝杆菌与胞内分枝杆菌之间的比较-”Int J Tuberc Lung Dis。

Satoko Hojo: "Severe cystic fibrosis associated with a △F508/R347H +D979A compound heterozygous genotype" Clin Genetics. 53. 50-53 (1998)

Satoko Hojo：“与 △F508/R347H +D979A 复合杂合基因型相关的严重囊性纤维化”《临床遗传学》53. 50-53 (1998)。

Yuka Obayash: "Successive follow-up of chest computed tomography in patietns with Mycobacterium avium-intracellulare complex"Respir Med. 93. 11-15 (1999)

Yuka Obayash：“对鸟分枝杆菌-细胞内复合体患者进行胸部计算机断层扫描的连续随访”Respir Med。