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The effect of steroid hormone on suppression of IgE production in the potients with bronchial astma and atopic dermatitis.

类固醇激素对支气管哮喘和特应性皮炎患者 IgE 产生抑制的作用。

基本信息

批准号：
06670803
负责人：
KURASHIGE Takanobu
金额：
$ 0.9万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

In the present study, we measured IFN-gamma production from peripheral blood mononuclear cells (PBMC) stimulated with IL-2 in asthmatic children with/without asthma attack, as well as serum cortisol level, in order to evaluate the correlation between asthma attack and endogenous cortisol level or IFN-gamma production.As results : (1) Both morning & evening serum cortisol level in patients with asthma attack was correlated with the severity of asthma attack. (2) Both morning & evening serum cortisol level in patients with asthma attack was significantly higher than that in patients without asthma attack. (3) In subject to subject study, serum cortisol level in patients with asthma attack was significantly higher than that in the same subject without asthma attack. (4) Exogenous corticosteroid inhibited IFN-gamma production from PBMC in a dose-dependent manner. (5) IFN-gamma production from PBMC stimulated with IL-2 in patients with asthma attack reversely correlated with the severity of … More the asthma attack. (6) IFN-gamma production from PBMC stimulated with IL-2 in patients with asthma attack was significantly lower than that without asthma attack. (7) In subject to subject study, IFN-gamma production from PBMC stimulated with IL-2 in patients with asthma attack was significantly lower than that in the same subject without asthma attack.These data suggest than endogenous cortisol level is elevated and IFN-gamma production in suppressed during asthma attack when compared without attack. As corticosteroid can inhibit IFN-gamma production in vitro, the suppression of IFN-gamma production during asthma attack is thought to be at least in part due to the elevation of endogenous cortisol.In addition, exogenous corticosteroid inhibited IL-4 and IL-2 production from PBMC in a dose dependent manner, like IFN-gamma production in vitro.In the future study, we well measure IFN-gamma, IL-4, IL-2 and IL-5 production from PBMC stimulated with Con A,that may induce the production of these cytokines simultaneously, in patients with atopic dermatitis (treated with/without tipical steroid, or treated with deep sea water) asthma (with/without attack). The influence of topical steroid or deep sea water IgE production or other immune response will be examined. Less

在本研究中，我们检测了哮喘发作和未发作的哮喘儿童外周血单个核细胞（PBMC）在IL-2刺激下产生的IFN-γ，以及血清皮质醇水平，以评估哮喘发作与内源性皮质醇水平或IFN-γ产生的相关性。（1）哮喘发作患者早晚血清皮质醇水平与哮喘发作的严重程度相关。(2)哮喘发作组晨、晚血清皮质醇水平均显著高于非发作组。(3)在个体间研究中，哮喘发作患者的血清皮质醇水平显著高于同一受试者的非哮喘发作水平。(4)外源性皮质类固醇以剂量依赖性方式抑制PBMC产生IFN-γ。(5)哮喘发作期IL-2刺激PBMC产生IFN-γ与哮喘严重程度相关 ...更多信息哮喘发作(6)哮喘发作患者经IL-2刺激后PBMC产生IFN-γ的水平显著低于未发作患者。(7)在个体间研究中，哮喘发作患者PBMC经IL-2刺激后产生IFN-γ的水平显著低于未发作的同一受试者，这些数据表明，与未发作相比，哮喘发作期间内源性皮质醇水平升高，IFN-γ的产生受到抑制。由于皮质类固醇在体外可抑制IFN-γ的产生，因此认为哮喘发作时IFN-γ产生的抑制至少部分是由于内源性皮质醇的升高。此外，外源性皮质类固醇以剂量依赖的方式抑制PBMC产生IL-4和IL-2，就像IFN-γ在体外的产生一样。在患有特应性皮炎（用/不用噻托溴铵类固醇治疗，或用深海水治疗）哮喘（有/无发作）的患者中，用Con A刺激PBMC产生IL-2和IL-5，这可能同时诱导这些细胞因子的产生。将检查局部类固醇或深海水IgE产生或其他免疫应答的影响。少