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THE ROLES OF INTERLEUKINS IN THE PATHOGENESIS OF HEMPHAGOCYTIC SYNDROME

白细胞介素在噬血细胞综合征发病机制中的作用

基本信息

批准号：
06670817
负责人：
HIBI Shigeyoshi
金额：
$ 1.34万
依托单位：
KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670817/
关键词：
Hemophagocytic syndrome cytokines EB virus IL 12 T cells NK cells NK細胞 Hemophagocytic Syndrome 血球貪食症候群 Hemophagecytic Syndrome

项目摘要

During the term of project (1994.4.1-1996.3.31). we have investigated hemophagocytic syndrome (HPS) using samples (peripheral blood mononuclear cells, bone marrow cells, lymphnode cells, spleen cells, and liver cells) obtained from 43 cases of this disease. Cellular phenotype was analyzed by flow cytometer and immunocytochemistry. Clonality was studied by chromosomal analysis, and Southern analysis of T cell receptor and immunoglobulin gene rerrangenment, and EB viral genome. Serum levels of various kinds of cytokines (IFN-g, IL-6, soluble IL2 receptor, IL-12) at active and inactive phase were measured by ELIZA or immunoradioassay.The results showed that EB viral involvement was demonstrated in 16 cases (38%). Of the 16 cases, two cases were T cell lineage, 6 cases were NK cell lineage, and 4 cases were T cell or NK cell lineage. Because od shortage of samples lineage was not identified in 4 cases, and the rest one case was reactive HPS.Of the EB virus associated 16 HPS cases, 11 cases were died of disease (69%), which clearly showed that this type of disease has quite poor prognosis. Serum IFN-g.IL-6, soluble IL-2 receptor levels in active HPS were extremely high regardless of EB viral involvement. However, IFN-g was especially high in EB virus associated HPS (9.2-330U/ml, normal<0.1U/ml), while its serum levels in infections mononucleosis (IM).which is also caused by EB viral infection, was below 3.0U/ml. We also analyzed serum IL12 levels in 29 cases of HPS and 4 cases of IM by establishing ELIZA measuring system. IL12 level was elevated in 5 case of HPS (1.4-22.5ng/ml). This may indicates that IL-12 is involved in a certain group of EB virus associated HPS.

项目期间（1994.4.1-1996.3.31）。我们使用从43例噬血细胞综合征（HPS）患者中获得的样本（外周血单核细胞、骨髓细胞、淋巴结细胞、脾细胞和肝细胞）研究了HPS。流式细胞仪和免疫细胞化学分析细胞表型。通过染色体分析、T细胞受体和免疫球蛋白基因重排以及EB病毒基因组的Southern分析来研究克隆性。用ELISA法或放射免疫法测定活动期和非活动期血清中各种细胞因子（IFN-γ、IL-6、可溶性IL-2受体、IL-12）的水平，结果显示16例（38%）有EB病毒感染。16例中，T细胞系2例，NK细胞系6例，T细胞或NK细胞系4例。4例因标本缺乏而未能确定家系，另1例为反应性HPS，在EB病毒相关的16例HPS中，有11例（69%）死于疾病，表明该类型疾病预后极差。活动性HPS的血清IFN-γ、IL-6、可溶性IL-2受体水平极高，与EB病毒的参与无关。而EB病毒感染引起的单核细胞增多症（IM）患者血清IFN-γ水平低于3.0U/ml。并建立ELISA检测系统，检测29例HPS和4例IM患者血清IL 12水平。5例HPS患者IL 12水平升高（1.4-22.5ng/ml）。提示IL-12可能参与了一组EB病毒相关的HPS。