The role of protease in pathogenesis of psoriasis

蛋白酶在银屑病发病机制中的作用

• 批准号: 06670881
• 负责人: TAKAMORI Kenji
• 金额: $ 1.34万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670881/
• 关键词: psoriasis; cell cycle; keratinocyte; keratinazation; proteasome; cyclin; cell cycle; プロテアーゼ; 病像形成; 表皮細胞

It is well known that the cell cycle of epidermal cell in involved skin of patient with psoriasis is shortend remarkably. The shortening of the cell cycle of epiderma1 cell is an important event in the pathogenesis of psoriasis, However the mechanisms of the shortening of the cell cycle are not obscure. In this report, we examined at first the distribution of non-lysosomal proteinase such as proteasome and cyclin in skin, and then investigated the role of proteasome in epidermal keratinization.1. Distribution of proteasome in epidermisImmunochemical staining showed considerably increased expression of proteasome in the cytoplasm and the nuclei in both normal and psoriasis skin. Proteasome activity also showed highest activity in living layer(granular-spinous layer).2. Distribution of cyclin proteinCyclin A was not stained in normal epidermis, however, stained stained strongly in psoriasis epidermis. Cyclin D was not detected in normal and psoriasis epidermis.3. The role of proteasome in keratinazationProteasome increased in both protein level and mRNA level time dependently in proportion to the degree of keratinazat ion.These results suggest that proteasome is related to the keratinazatio in epidermis and cyclin A is involved in the proliferation of epidermis.

众所周知，银屑病患者受累皮肤表皮细胞的细胞周期明显缩短。表皮细胞周期的缩短是银屑病发病过程中的一个重要事件，但其缩短的机制尚不清楚。在这篇报道中，我们首先检查了非溶酶体蛋白酶如蛋白酶体和细胞周期蛋白在皮肤中的分布，然后研究了蛋白酶体在表皮角化中的作用。表皮中蛋白酶体的分布免疫化学染色显示，在正常皮肤和银屑病皮肤中，胞质和细胞核中蛋白酶体的表达明显增加。蛋白酶体活性在活层（颗粒-棘层）中最高。细胞周期蛋白A在正常表皮中未被染色，但在银屑病表皮中染色强烈。正常和银屑病表皮均未检出Cyclin D。蛋白酶体在角化过程中的作用蛋白酶体的蛋白水平和mRNA水平随角化程度呈时间依赖性增加。这些结果表明蛋白酶体与表皮角化有关，细胞周期蛋白A参与表皮的增殖。