NEUROCHEMICAL STUDIES OF PATHOPHYSIOLOGY OF SCHIZOPHRENIA IN THE HIPPOCAMPAL REGION.

海马区精神分裂症病理生理学的神经化学研究。

基本信息

批准号：
06670955
负责人：
KURUMAJI Akeo
金额：
$ 1.41万
依托单位：
TOKYO MEDICAL AND DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Systemic administration of DOI (1mg/kg, sc) incuced an increase in numbers of head shaking in rats lesioned bilateral entorhinal cortex, compared to those of sham-operated rats. These results indicate that the lesion of entorhinal cortex stimulates neuraltransmisssions mediated through 5HT-2 receptors in certain brain area associated with DOI-induced head shaking.[^<125>I] iomazenil bindings, labeling the central-type benzodiazepine receptors in 37 discrete rat brain areas were measured after single (7.5mg/kg, i.p) and repeated (7.5mg/kg*14 days, i.p.) treatment with phencyclidine (PCP), a noncompetetive antagonist of NMDA receptors, using in vitro quantative autoradiographic receptor binding assay. A significant increase in [^<125>I] iomazenil bindings was observed in the superficial layr of the parietal cortex in both of the PCP treatmnet groups and the CA1 of the hippocampus of the repeated PCP-treated group. There was a significant decrease in the binding in the piriform cortex of the repeated PCP-treated group. These results suggest that the blockade of NMDA receptor by PCP produces the compensational alterations in the central-type benzodiazepine receptor antagonist binding.The concentartions of glycine and its potential precursors in 20 areas of the postmortem brains of chronic schizophrenics and controls were measured by high-performance liquid chromatography. Increased contents of glycine and serine were observed in the orbitofronatal cortex of schizophrenics, suggesting that an abnormal metabolism between glycine and serine may be involved in the pathophysiology of schizophrenia.

与假手术大鼠相比，全身施用DOI（1mg/kg，SC）在大鼠双侧肠胃皮质中促进了头部颤抖数量的增加。这些结果表明，内嗅皮层的病变刺激通过5HT-2受体在某些大脑区域中介导的神经发射剂，与doi诱导的头部摇动相关。[^<125> i] iomazenil结合物，标记了中央型苯甲二氧化氮受体，在37个Invection Rage the Indivepe type type type type and in Single（kg）中。（7.5mg/kg*14天，i.p.）使用体外量化自显影受体结合测定法（NMDA受体的非竞争力拮抗剂）苯克林（PCP）治疗。在PCP处理组的两个顶叶皮层的表层和重复PCP治疗组的海马的CA1中，观察到[^<125> i] iomazenil结合的[^<125> i] iomazenil结合。重复的PCP治疗组的梨状皮层中的结合显着下降。这些结果表明，PCP对NMDA受体的阻断产生了中央型苯并二氮卓受体拮抗剂结合的补偿性改变。甘氨酸的浓度及其在慢性精神分裂症和对照组的20个区域的20个区域的潜在前体通过高强度的液体液体液体液体照相来测量。在精神分裂症患者的轨道上皮层中观察到甘氨酸和丝氨酸的含量增加，这表明甘氨酸和丝氨酸之间的异常代谢可能与精神分裂症的病理生理有关。