Mechanisms for the formation of granulocytic sarcoma in leukemia

据报道，胶原酶等金属蛋白酶的产生与癌细胞的转移有关。髓外部位的粒细胞肉瘤的形成机制与其他癌症相似。然而，白血病髓外肿瘤的形成机制尚不清楚。以前，有报道称骨髓单核细胞分泌92 kDa的IV型胶原酶，并像肿瘤细胞一样穿过基底膜，因此。我们检测了几种人类白血病细胞系的IV型胶原酶的分泌，包括从一例真皮组织粒细胞肉瘤患者建立的粒细胞肉瘤来源的细胞系。我们还检测了这些白血病细胞系对重组基底膜的侵袭能力。马特里格尔。用于体外侵袭实验。在白血病细胞系中，只有粒细胞肉瘤细胞系分泌IV型胶原酶，据报道，IV型胶原酶具有降解IV型胶原酶的作用。其他髓系白血病细胞系，如HL-60和U-937，只有在用12-0-十四烷基酚-13-乙酸酯处理后才能产生IV型胶原酶。在体外侵袭实验中，粒细胞肉瘤细胞株表现出比其他细胞株更强的侵袭能力，提示92 kDa IV型胶原酶的分泌在白血病髓外肿瘤的形成中起重要作用。

Production of metalloproteinases such as collagenases has been reported to be involved in the metastasis of cancer cells. Granulocytic sarcoma inextramedullary sites can be formed by similar mechanisms to other cancers. However the mechanisms of extramedullary tumor formation in leukemia have not been clarified. Previously myelomonocytic cells have been reported to secrete 92 kDa type IV collagenase and to traverse the basement membrane as tumor cells do.Therefore. we examined the secretion of type IV collagenases in several human leukemia cell lines, including a granulocytic sarcoma-derived cell line established from a patient with granulocytic sarcomas in dermal tissues. We also examined the invasiva capacity of these leukemia cell lines into reconstituted basement membrane. Matrigel. which was used for in vitro invasion assay. Among the leukemia cell lines, only the ranulocytic sarcoma cell line secretes type IV collagenase, which has been reported to degrade type IV collagenase, constitutively. Other myeloid leukemia cell lines such as HL-60 and U-937 produce type IV collagenase only after being treated with 12-0-tetradecanoylphorvol-13-acetate. The granulocytic sarcoma cell line shows higher invasive capacity than the other cell lines in in vitro invasion assay.These results suggest than the secretion of 92 kDa type IV collagenase plays a role in extramedullary tumor formation in leukemia.