Serum factors from pancreas cancer patients, which enhance induction of k-ras oncogene mutation

胰腺癌患者的血清因子可增强 k-ras 癌基因突变的诱导

• 批准号: 06671183
• 负责人: YAMAMORI Hideo
• 金额: $ 1.22万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671183/
• 关键词: Pancreas cancer; Serum factors; K-ras; Oncogene; Mutation; K‐ras; 突然変異誘発因子; 形質変異

Serum factors which enhance induction of K-ras oncogene mutation were searched for in sera from pancreas cancer patients. Sera were fractionated by chromatography and tested for the activity to enhance protease-inducibility in lymphocytes from healthy donors. Fractions with the enhancement were examined with respect to the activity to enhance induction of phenotypic mutation in cultured human cells, following the examination of the activity to enhance induction of base substitution mutations of K-ras codon 12 in human cells. Sera from one patient were fornd to have the enhancement activity on phenotypic and genetic mutation. Results may indicate that high incidence of K-ras codon 12 mutations in pancreas cancer is partly due to enhancement of the mutation by the serum factors found here.

从胰腺癌患者血清中寻找促进K-ras癌基因突变诱导的血清因子。用层析法对血清进行分离，并测试其增强健康供者淋巴细胞中蛋白水解酶诱导性的活性。在检测了增强K-ras密码子12碱基替换突变的活性之后，检测了具有增强活性的组分在培养的人类细胞中增强诱导表型突变的活性。1例患者血清对表型和基因突变均有增强作用。结果提示胰腺癌K-ras密码子12突变的高发部分是由于血清因子对该突变的促进作用。

项目成果

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N.Suzuki,H.Yamamori,et al.: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines." Jpn.J.Cancer Res.86. 761-769 (1995)

N.Suzuki、H.Yamamori 等人：“双嘧达莫与肿瘤坏死因子-α 结合可增强对人肿瘤细胞系增殖的抑制。”Jpn.J.Cancer Res.86 (1995)。

K.Takagi, H.Yamamori et al: "Recombinant human growth hormone and protein metabolism of burned rats and esophagectomized patients." Nutrition. 11. 22-26 (1995)

K.Takagi、H.Yamamori 等人：“烧伤大鼠和食管切除患者的重组人生长激素和蛋白质代谢。”