Serum factors from pancreas cancer patients, which enhance induction of k-ras oncogene mutation

在胰腺癌患者血清中寻找可增强诱导K-ras癌基因突变的血清因子。用层析法分离血清，检测其增强健康供体淋巴细胞蛋白酶诱导能力的活性。在对人类细胞中K-ras密码子12碱基置换突变的诱导活性进行检测之后，对具有增强作用的组分在培养的人类细胞中增强诱导表型突变的活性进行了检测。1例患者血清对表型和基因突变均有增强作用。结果可能表明，胰腺癌中K-ras密码子12突变的高发生率部分是由于血清因子增强了突变。

Serum factors which enhance induction of K-ras oncogene mutation were searched for in sera from pancreas cancer patients. Sera were fractionated by chromatography and tested for the activity to enhance protease-inducibility in lymphocytes from healthy donors. Fractions with the enhancement were examined with respect to the activity to enhance induction of phenotypic mutation in cultured human cells, following the examination of the activity to enhance induction of base substitution mutations of K-ras codon 12 in human cells. Sera from one patient were fornd to have the enhancement activity on phenotypic and genetic mutation. Results may indicate that high incidence of K-ras codon 12 mutations in pancreas cancer is partly due to enhancement of the mutation by the serum factors found here.