EXPERIMENTAL STUDY ON ISCHEMIA-REPERFUSION INJURY OF CIRRHOTIC LIVER -INFLUENCE OF GRADE OF HEPATIC CIRRHOSIS-

1.研究了硫代乙酰胺诱导的肝硬化（TAA-liver）和正常肝对缺血性损伤的耐受性。大鼠肝脏缺血60分钟。(1)TAA肝的一周存活率显著高于正常肝。(2)再灌注60 min后，TAA肝胆汁流量及胆汁流量恢复率明显低于正常肝。(3)再灌注60分钟后，TAA肝ATP含量明显低于正常肝。(4)再灌注60分钟后，TAA-肝中TAN浓度显著低于正常肝。(5)再灌注60分钟后，TAA肝的能荷明显低于正常肝。2.比较了连续60分钟肝缺血（C组）和间断性肝缺血（I组）对大鼠TAA肝的影响。(1)C组1周生存率明显高于I组。(2)再灌注后60 min，C组胆汁流量及胆汁流量恢复率明显低于I组。(3)再灌注60 min后，C组ATP含量明显低于I组。(4)再灌注60 min后C组TAN浓度明显低于I组。(5)再灌注60分钟后，C组的能量负荷明显低于I组。因此，当TAA-肝脏需要60分钟的缺血时，间歇性短暂缺血是有用的。

1.We studied the tolerance to ischemic injury of thioacetoamido-induced cirrhotic liver (TAA-liver) and normal liver. Rats underwent hepatic ischemia for 60 minutes. (1) One week survival in TAA-liver was significantly greater than that in normal liver. (2) Sixty minutes after the reperfusion, bile flow and recovery rate of bile flow in TAA-liver was significantly lower than those in normal liver. (3) Sixty minutes after the reperfusion, ATP concentration in TAA-liver was significantly lower than those in normal liver. (4) Sixty minutes after the reperfusion, TAN concentration in TAA-liver was significantly lower than those in normal liver. (5) Sixty minutes after the reperfusion, energy charge in TAA-liver was significantly lower than those in normal liver. These data suggested that tolerance to ischemic injury should be much lower in TAA-liver than those in normal liver.2.We compared the influence of continuous 60 minutes hapatic ischemia (group C) and intermittent hapatic ischemia (four times of 15 minutes of ischemia with five minutes intervals : group I) on TAA-liver in rats. (1) One week survival in group C was significantly greater than that in group I.(2) Sixty minutes after the reperfusion, bile flow and recovery rate of bile flow in group C was significantly lower than those in group I.(3) Sixty minutes after the reperfusion, ATP concentration in group C was significantly lower than those in group I.(4) Sixty minutes after the reperfusion, TAN concentration in group C was significantly lower than those in group I.(5) Sixty minutes after the reperfusion, energy charge in group C was significantly lower than those in group I.We might conclude that intermittent brief ischemia was useful when 60minutes of ischemia was required for the TAA-liver.