EXPERIMENTAL STUDY ON ISCHEMIA-REPERFUSION INJURY OF CIRRHOTIC LIVER -INFLUENCE OF GRADE OF HEPATIC CIRRHOSIS-

肝硬化缺血再灌注损伤的实验研究-肝硬化分级的影响-

• 批准号: 06671261
• 负责人: SENGA Shoshi
• 金额: $ 1.15万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671261/
• 关键词: Thioacetoamido-induced cirrhotic liver; Liver cirrhosis; intermittent brief ischemia; Bile flow; Hepatic ischemia; ischemia-reperfusion injury; High energy phosphate; チオアセトアシド肝硬変; 脂肪性肝硬変; 肝虚血再灌流障害; 高エネルギーりん酸化合物

1.We studied the tolerance to ischemic injury of thioacetoamido-induced cirrhotic liver (TAA-liver) and normal liver. Rats underwent hepatic ischemia for 60 minutes. (1) One week survival in TAA-liver was significantly greater than that in normal liver. (2) Sixty minutes after the reperfusion, bile flow and recovery rate of bile flow in TAA-liver was significantly lower than those in normal liver. (3) Sixty minutes after the reperfusion, ATP concentration in TAA-liver was significantly lower than those in normal liver. (4) Sixty minutes after the reperfusion, TAN concentration in TAA-liver was significantly lower than those in normal liver. (5) Sixty minutes after the reperfusion, energy charge in TAA-liver was significantly lower than those in normal liver. These data suggested that tolerance to ischemic injury should be much lower in TAA-liver than those in normal liver.2.We compared the influence of continuous 60 minutes hapatic ischemia (group C) and intermittent hapatic ischemia (four times of 15 minutes of ischemia with five minutes intervals : group I) on TAA-liver in rats. (1) One week survival in group C was significantly greater than that in group I.(2) Sixty minutes after the reperfusion, bile flow and recovery rate of bile flow in group C was significantly lower than those in group I.(3) Sixty minutes after the reperfusion, ATP concentration in group C was significantly lower than those in group I.(4) Sixty minutes after the reperfusion, TAN concentration in group C was significantly lower than those in group I.(5) Sixty minutes after the reperfusion, energy charge in group C was significantly lower than those in group I.We might conclude that intermittent brief ischemia was useful when 60minutes of ischemia was required for the TAA-liver.

1.研究了硫代乙酰胺诱导的肝硬变(TAA-肝)和正常肝对缺血损伤的耐受性。大鼠肝脏缺血60min。(1)移植肝1周存活率显著高于正常肝。(2)再灌流60min后，TAA肝的胆汁流量和胆汁流量恢复率明显低于正常肝。(3)再灌流60min后，TAA肝组织中的ATP含量明显低于正常肝组织。(4)再灌流60min后，TAA肝组织中TAN含量明显低于正常肝组织。(5)再灌流60min后，TAA肝组织能荷显著低于正常肝组织。2.比较了连续60min肝缺血(C组)和间歇性肝缺血(I组)对大鼠TAA肝的影响。(2)再灌流60min后，C组胆汁流量及胆汁流量恢复率明显低于I组。(3)再灌流60min，C组三磷酸腺苷浓度明显低于I组。(4)再灌流60min，C组Tan浓度显著低于I组。C组的能荷显著低于I组。当TAA-肝脏需要缺血60min时，间歇性短暂缺血是有用的。

项目成果

Hirokazu Matsutomo, Hajime Hirose, Shoshi Senga, Hideo Itoh, Masatoshi Hayashi, Yoshihumi Katagiri, Tatsumi Iida, Masatomo Hayashi, Atsuyoshi Onitsuka: "An experimental stydy on tolerance to ischemic injury of thioacetamide-induced liver cirrhosis in rat"

Hirokazu Matsutomo、Hajime Hirose、Shoshi Senga、Hideo Itoh、Masatoshi Hayashi、Yoshihumi Katagiri、Tatsumi Iida、Masatomo Hayashi、Atsuyoshi Onitsuka：“大鼠硫代乙酰胺诱导的肝硬化缺血性损伤耐受性的实验研究”

松友 寛和: "Thioacetamideによるラット硬変肝の虚血耐容能に関する実験的検討" 日本消化器外科学会雑誌掲載予定. 29. (1996)

松友博和：“硫代乙酰胺诱导的大鼠肝硬化缺血耐受性的实验研究”预定发表在日本胃肠外科学会杂志29号上。（1996年）