Basic and Clinical Studies for the Relationship between Multiple Organ Failure and Intestinal Mucosal Injury.

多器官衰竭与肠粘膜损伤关系的基础和临床研究。

• 批准号: 06671263
• 负责人: MIYACHI Masahiko
• 金额: $ 1.28万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671263/
• 关键词: ischemic; reperfusion injury; intestinal blood flow; bacterial translocation; operation; nitric oxide; L-NAME; endotoxin; 小腸粘膜障害; Bacterial translocation; 食道切除術; 小腸虚血再灌流障害; 小腸虚血再灌流; 小腸組織血流; xanthine oxidase活性

1) In the anesthetised dog, the intestinal blood flow measured with the laser doppler flowmetry increased during reperfusion after 5-minutes intestinal iscemia more than before the intestinal ischemia. This increased blood flow was inhibited by a intravenous infusion of nitric oxide synthetase blocker, L-NAME.2) Serum endotoxin concentrations after thoracotomy increased more than after laparotomy.3) The blood flow of the jejunum decreased during thoracotomy, especially during cardiac compression more than during laparotomy. However, when the blood flow of the jejunum decreased during thoracotomy, not only systemic blood pressure but also cardiac output did not decrease remarkablly.4) These results suggest that intensive operative stress, such as cardiac compression, cause the increase of the splanchnic asterial resistance and the decrease of the blood flow of the small intestine and then these phenomena may cause the intestinal mucosal injury and induce bacterial translocation in the intestinal wall.

1)麻醉犬在肠缺血5分钟后再灌注时，激光多普勒血流仪测量的肠血流量比肠缺血前增加。静脉输注一氧化氮合成酶阻滞剂L-NAME可抑制血流增加。2)开胸术后血清内毒素浓度高于剖腹术后。3)开胸时空肠血流量明显减少，尤其是心脏压迫时，空肠血流量明显减少。然而，当开胸时空肠血流量减少时，不仅全身血压下降，心排血量也没有明显下降。4)以上结果提示，心脏压迫等高强度手术应激可引起肠壁阻力增加，小肠血流量减少，从而引起肠黏膜损伤，引起肠壁细菌易位。

项目成果

Aono K.at al: "Kupffer cells cytotoxicity against hepatoma cells isrelated to nitric oxide." Biochem Biophys Res Commun. 201. 1175-1181 (1994)

Aono K.at al：“Kupffer 细胞对肝癌细胞的细胞毒性与一氧化氮有关。”

Kato M.et al.: "Intravenous catecholamine alter hepatic blood flow in concious dogs with experimental hepatic denervation." Journal of Surgical Research. 66. 179-184 (1996)

Kato M.等人：“在实验性肝去神经术中，静脉注射儿茶酚胺会改变清醒狗的肝血流量。”

Akiyama H at al: "Hepatic denervation induced supersensitivity to terbutaline of the hepatic arterial system in concious dogs." Journal of Surgical Research. 68. 67-72 (1997)

Akiyama H 等人：“去肝神经支配会导致有意识的狗的肝动脉系统对特布他林产生超敏感性。”

Aono K.et.al.: "Kupffer cells cytotoxicity against hepatoma cells is related to nitric oxide." Biochem Biophys Res Commun. 201. 1175-1181 (1994)

Aono K.et.al.：“库普弗细胞对肝癌细胞的细胞毒性与一氧化氮有关。”

Akiyama H.at.al.: "Hepatic denervation induced supersensitivity to terbutaline of the hepatic arterial system in concious dogs." Journal of Surgical Research. 68. 67-72 (1997)

Akiyama H.at.al.：“去肝神经支配会导致有意识的狗的肝动脉系统对特布他林产生超敏感性。”