An experimental study of enhanced anticancer effect of intraperitoneal chemotherapy with high energy shock waves

体外观察顺铂腹腔注射抗肿瘤活性。将Erhlich腹水癌细胞悬浮于含5-50杯/ml顺铂的生理盐水中30分钟，测定细胞内对顺铂的摄取。依氏细胞吸收的顺铂积累量与顺铂浓度成比例增加。10杯/毫升顺铂溶液中顺铂的细胞摄取按时间顺序增加。然后研究腹腔注射顺铂和体外低水平超声对小鼠体内的影响。通过腹腔注射Erhlich细胞诱导小鼠腹膜癌变。在细胞注射后24和94小时，以5 mg/kg的剂量给予顺铂溶液50 mg/ ml。超声波（28khz）照射小鼠3分钟、5分钟或10分钟。在细胞注射后24小时处理小鼠时，未处理组的中位生存天数分别为11.5、14.5、14.0、21.5和23天，分别为单药组、3分钟超声波组、5分钟超声波组和10分钟超声波组。腹腔注射顺铂和5、10分钟超声治疗组荷瘤小鼠的生存期明显延长（P<0.01， Cox-Mantel检验）。注射细胞后96 h，小鼠的中位生存天数分别为：不治疗组、单用顺铂组、超声波3分钟组、超声波5分钟组和超声波10分钟组的中位生存天数分别为12.0、16.0、15.5、22.0和24.5天。腹腔注射顺铂和5、10分钟超声治疗比不治疗和单独使用顺铂组的小鼠生存期明显延长（P<0.01， Cox-Mantel检验）。提示腹腔顺铂联合体外低水平超声治疗腹膜播散比单用顺铂治疗更有效。少

Antitumor activity of intraperitoneal cisplatin was examined in vitro. Erhlich ascites carcinoma cells were suspended in phyisiolosic saline which contained 5-50 mug/ml of cisplatin for 30 minutes, and intracellular uptake of cisplatin was measured. The accumulation of cisplatin taken up by Erhlich cells increased in proportion to the concentration of cisplatin. The cellular uptake of cisplatin in 10 mug/ml cisplatin solution increased chronologically. Then in vivo effects of intraperitoneal cisplatin and extracorporeal low-level ultrasonic waves were studied. A peritoneal carcinomatosis was induced in ddy mice by intraperitoneal injection of Erhlich cells. At 24 and 94 hours after cell injection, cisplatin solution (50 mug/ml) was administered at a dose of 5 mg/kg to ddy mice. Ultrasonic waves (28 kHz) were irradiated to mice for 3,5, or 10 minutes. When mices were treated at 24 hours after cell injection, the median survival days were 11.5,14.5,14.0,21.5 and 23 in the no-treatment, c … More isplatin alone, 3-minute ultrasonic waves, 5-minute ultrasonic waves and 10-minutes ultrasonic waves, respectively. The survival of intraperitoneal tumor-bearing ddy mice was greatly prolonged by intraperitoneal cisplatin and 5- or 10-minute ultrasonic waves as compared with those of the no-treatment or cisplatin aalone (P<0.01, Cox-Mantel test). When mices were treated at 96 hours after cell injection, the median survival days were 12.0 16.0,15.5,22.0 and 24.5 in the no-treatment, cisplatin alone, 3-minute ultrasonic waves, 5-minute ultrasonic waves and 10-minutes ultrasonic waves, respectively. The survival of intraperitoneal tumor-bearing ddy mice was greatly prologned by intraperitoneal cisplatin and 5- or 10-minute ultorasonic waves as compared with those of the no-treatment or cisplatin alone (P<0.01, Cox-Mantel test). These results indicate that the combination of intraperitoneal cisplatin and extracorporeal low-level ultrasonic waves may prevent and treat the peritoneal dissemination more effectively than the treatment of intraperitoneal cisplatin alone. Less