Peritoneal dissemination of gastric cancer stem cells and immunotherapy overcoming immune escape

胃癌干细胞的腹膜传播和克服免疫逃逸的免疫治疗

• 批准号: 23591939
• 负责人: TSUJITANI Shunichi
• 金额: $ 3.24万
• 依托单位: 独立行政法人国立国際医療研究センター (2012-2013)Kyushu University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591939/
• 关键词: がん幹細胞; 免疫回避機構; 樹状細胞; 免疫抑制; 胃癌; 腹膜播種

Peritoneal dissemination of gastric cancer stem cells and immunotherapy overcoming immune escape were investigated. Cultured gastric cancer cell lines MKN45 and MKN74 were sorted by FACS int0 CD44+ and CD44- cells. CD44+ gastric cancer cells expressed TGF-b , IL-10 and VEGF. Therefore, gastric cancer stem cells may have high potential to escape from immune surveillance. However, CTL from healthy donor co-cultured with CD44+ gastric cancer cells could kill CD44+ gastric cancer cells as well as that CTL co-cultured with CD44- gastric cancer cells could kill CD44- gastric cancer cells. These results indicate that cancer stem cells may not be resistant to immunotherapy but the cancer stem cells may be protected by strong escape mechanisms provided by host cells.

研究胃癌干细胞腹膜播散和免疫治疗克服免疫逃逸。培养的胃癌细胞系MKN 45和MKN 74通过FACS int 0 CD 44+和CD 44-细胞分选。CD 44+胃癌细胞表达TGF-β、IL-10和VEGF。因此，胃癌干细胞可能具有很高的潜力，以逃避免疫监视。与CD 44+胃癌细胞共培养的健康人CTL对CD 44+胃癌细胞有杀伤作用，与CD 44-胃癌细胞共培养的健康人CTL对CD 44-胃癌细胞也有杀伤作用。这些结果表明，癌症干细胞可能对免疫疗法没有抗性，但癌症干细胞可能受到宿主细胞提供的强逃逸机制的保护。

项目成果

Dendritic Cell-Based Immunotherapy Targeting Synthesized Peptides for Advanced Biliary Tract Cancer

• DOI: 10.1007/s11605-013-2286-2
• 发表时间: 2013-09-01
• 期刊: JOURNAL OF GASTROINTESTINAL SURGERY
• 影响因子: 3.2
• 作者: Kobayashi, Masanori;Sakabe, Tomoyo;Nagaya, Masaki
• 通讯作者: Nagaya, Masaki