权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Experimental study of protective of in-situ hypothermic liver perfusion on the ischemic liver with biliary obstruction in pigs

原位低温肝灌注对猪缺血性肝胆道梗阻保护作用的实验研究

基本信息

批准号：
06671280
负责人：
ASAHARA Toshimasa
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

To minimize the damage during total hepatic vascular exclusion and after reperfusion, in-situ cold perfusion via portal vein and hepatic artery was carried out in pig for clinical application to liver surgery of hepatocellular carcinoma and cholangiocellular carcinoma with liver cirhosis and obstructive jaundice.1. Study of the usefulness of V-V bypass and cold perfusion of the liverIn-situ hypothermic perfusion could maintain hepatic TAN level higher than that of warm ischemia group during and sfter HVE (hepatic vascular exclusion). In-situ hypothermic perfusion could prevent the damage caused by liver ischemia.2. Study of the usefulness of V-V bypass and cold perfusion of the liver with obstructive jaundiceMore remarkable liver cell injury was induced by warm ischemia in pig with obstructive jaundice, and in-situ hypothermic perfusion of the liver could prevent the damages during and after liver ischemia not only in normal liver but also in the liver with obstructive jaundice.3. Study for the possibility of prologation of V-V bypass and cold perfusoin of the liverThe in-situ hypothermic perfusion technique is useful for reducing ischemia-reperfusion injury, and especially could protect the liver injury from 180 minutes ischemia of the liver with UW solution.4. Study for the protective effect of PGI2 analogue (OP2507) on liver ischemic injuryPGI2 analogue has a cytoprotective effect and maintains high energy metabolism of the liver against ischemic reperfusion injury. Administration of PGI2 analogue with in-situ liver cold perfusion could prevent the damage caused by prolonged liver ischemia.

为最大限度地减少全肝血流阻断时和再灌流后的损伤，本实验通过门静脉和肝动脉对猪进行原位冷灌流，以应用于肝细胞癌和胆管细胞癌合并肝硬变和梗阻性黄疸的肝外科手术。肝血流阻断前后原位低温灌流可使肝脏TAN水平维持在高于热缺血组的水平。结论：1.原位低温灌流可预防肝脏缺血损伤。梗阻性黄疸猪肝脏热缺血后肝细胞损伤更为明显，原位低温肝脏灌流不仅可以预防梗阻性黄疸大鼠肝脏缺血过程中和缺血后的损伤，而且对梗阻性黄疸大鼠的肝细胞损伤也有一定的保护作用。探讨延长V-V转流和肝脏冷灌流的可能性原位低温灌流技术有利于减轻肝脏缺血再灌注损伤，尤其是UW液对肝脏缺血180分钟所致的肝脏损伤具有保护作用。前列环素类似物(OP2507)对肝脏缺血损伤保护作用的研究前列环素类似物具有细胞保护作用，可维持肝脏对缺血再灌注损伤的高能量代谢。在肝原位冷灌流的同时给予PGI2类似物可预防肝脏长时间缺血所致的损伤。