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Trehalose-containing solutions enhance preservation of cultured endothelial cells.

含海藻糖的溶液可增强培养内皮细胞的保存。

基本信息

批准号：
06671339
负责人：
INUI Kenji
金额：
$ 1.41万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671339/
关键词：
Endothelial cell MTT assay Trypan blue exclusion method ET-Kyoto solution IT-Kyoto solution UW solution Euro Collins solution IT-Kyoto液トレハロース dye exclusion法 MTT assay法 ET-KYOTO液(ET-K液)

项目摘要

Background and Methods : We have developed two new types of preservation solutions containing trehalose. ET-Kyoto solution (ET-K) is an extracellular type and IT-Kyoto solution (IT-K) is an intracellular type. In the present study we examined with in vitro assays the ability of ET-K,IT-K,Euro-Collins (EC) and University of Wisconsin (UW) solutions to preserve a murine endothelial cell line. The viability of cells stored in the solutions at 4ﾟC was determined by trypan blue exclusion and MTT assay.Results : Trypan blue exclusion : The viability after 48 hours of cold storage was 49.5<plus-minus>4.7% (mean<plus-minus>S.E.) in ET-K,59.5<plus-minus>0.7% in IT-K,29.2<plus-minus>2.5% in EC, and 55.3<plus-minus>7.6% in UW (ET-K or UW vs EC,p<0.05 ; IT-K vs EC,p<0.01). MTT assay : Absorbance values for cells after 48 hours of cold storage were 0.366<plus-minus>0.0066 (mean<plus-minus>S.E.) in ET-K,0.358<plus-minus>0.0044 in IT-K,0.336<plus-minus>0.011 in EC,and 0.362<plus-minus>0.0019 in UW (ET-K or UW vs EC,p<0.05). After 120 hours, absorbance values for cells were 0.303<plus-minus>0.0038 in ET-K,0.269<plus-minus>0.0034 in IT-K,0.186<plus-minus>0.011 in EC,and 0.265<plus-minus>0.0066 in UW (ET-K vs UW,p<0.05 ; ET-K vs IT-K, p<0.01 ; ETConclusions : As far as the ability to preserve a murine endothelial cell line at a low temperature was concerned, the ET-K solution was puperior to the UW solutions, the IT-K solution and UW solution were equal, and the ET-K and IT-K solutions were superior to the EC solution.

背景和方法：我们研制了两种新型的含有海藻糖的保存液。ET-京都溶液(ET-K)属于胞外型，IT-京都溶液(IT-K)属于胞内型。在本研究中，我们通过体外检测ET-K、IT-K、Euro-Collins(EC)和威斯康星大学(UW)溶液保存小鼠内皮细胞系的能力。结果：台盼蓝拒染法：冷藏48h后细胞存活率为49.5lt；正负4.7%(平均值+lt；+gt；S.E.)在ET-K中，IT-K占59.5%，EC占29.2%，UW占55.3%，UW占7.6%(ET-K或UW vs EC，p&lt；0.05；IT-K vs EC，p&lt；0.01)。四甲基偶氮唑盐比色法：细胞冷藏48小时后的吸光度为0.366；+-0.0066(平均值++-GT；S.E.)在ET-K中，IT-K的正负0.0044，EC的正负0.011，UW的0.362&lt；正负0.0019(ET-K或UW与EC，p&lt；0.0019)。120h后，ET-K、IT-K、EC、UW的吸光度分别为0.303、0.269、0.0034、0.186、0.265、0.0066(ET-K vs UW，p&lt；0.05；ET-K vs IT-K，p&lt；0.01；ET-K vs IT-K，p&lt；0.01；结论：就低温保存小鼠内皮细胞系的能力而言，ET-K液优于UW液，IT-K液与UW液相当，ET-K和IT-K液优于EC液。