Molecular analysis of mechanism to metastasis of lung cancer : especially cells motility and growth factor genes

肺癌转移机制的分子分析：特别是细胞运动和生长因子基因

• 批准号: 06671361
• 负责人: YASUDA Shin-ichi
• 金额: $ 1.34万
• 依托单位: Dokkyo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671361/
• 关键词: Lung cancer; Tumor metastasis; Cytokine; Molecular analysis; 増殖因子

As for invarsion and metastasis to local site of cancer cells with cancer patients, the inhibition becomes important theme by bringing major influence for prognosis. Recently, the growth factor producced tumor is reported related to the organs selectivity of cancer metastasis by a mouse tumor. Previously, we found a stimulant substance for immuno competent cells with production from human lung cancer cells, the same factor with related to metastasis of lung cancer, we thought that did participate in cell movement to metastasis process of lung cancer and the control of autocrine/paracrine growth factor with tumor local site, then we studied the role in metastasis as an indicator with gene of produced growth factor by molecular biolog. The seventeen cells lines of malignant tumor including 13 lung cancer cells was using.TGF-beta mRNA was expressed in seventeen cells lines of malignant tumor. The mRNA of cytokine IL-8, IL-1alpha and IL-6 was expressed 80%, 65% and 35%, respectively. Further we analyzed for related to tumor metastasis in those cells. E-cadoherin, CD44 and c-met mRNA was expressed in 13 lines, 8 lines and 12 lines, respectively. Inversive activity by matrigel was shown in 10 cell lines. And the inversive activity of those cells related to the adhesion for matrigel and activation of metalloprotinases.Those results suggest the numerous cytokines playd an effective role for tumor progression through the cell interactions in microenvironment.

对于癌症患者癌细胞的侵袭和局部转移，抑制成为重要主题，对预后有重要影响。最近有报道称，生长因子产生的肿瘤与小鼠肿瘤对肿瘤转移的器官选择性有关。以前，我们从人肺癌细胞中发现了一种免疫活性细胞的刺激物质，与肺癌转移相关的因子，我们认为它确实参与了肺癌细胞向转移过程的运动和自分泌/旁分泌生长因子对肿瘤局部位置的调控，然后用分子生物学的方法研究了产生的生长因子基因作为指标在转移中的作用。以包括13个肺癌细胞在内的17个恶性肿瘤细胞系为研究对象，在17个恶性肿瘤细胞系中表达了转化生长因子-βmRNA。细胞因子IL-8、IL-1α和IL-6的mRNA表达分别为80%、65%和35%。进一步，我们分析了这些细胞中与肿瘤转移相关的因素。E-钙粘附素、CD44和c-met分别在13个细胞株、8个细胞株和12个细胞株中表达。Matrigel在10个细胞系中表现出逆转活性。这些细胞的逆转活性与基质黏附和金属蛋白酶的激活有关。这些结果表明，大量的细胞因子通过微环境中的细胞相互作用在肿瘤的进展中发挥有效的作用。