New metastasis regulated genes from human lung carcinoma to functional analysis and application for therapy

原发肿瘤的转移是通过许多过程得出的。这是与患者预后密切相关的重要问题，也是对癌症治疗进行分析的重要问题。我们建立了肺癌细胞系，它只向肺转移。对转移细胞和非转移细胞进行减数处理。我们分析了四个与转移相关的基因。层粘连蛋白受体(LR)基因是细胞黏附蛋白与层粘连蛋白的特异性受体。分析结果表明，一个密码子作用于一个67 kDa分子的部分基因中存在Exson缺失。PAF-AH是血小板产生的磷脂激活因子(PAF)的乙酰水解酶，与肿瘤转移有关。它参与了神经细胞的运动，但癌症的转移尚不清楚。提示该PAF-AH基因在非转移系中的表达使PAF失活，从而参与肿瘤的转移控制。在此基础上，采用RACE方法对两个未知基因片段进行分析，获得了该延伸片段的基因序列。这些基因都与黑色素瘤有关，其中一个存在于14号染色体上，与宫颈癌和肠粘膜蛋白有关，最近被基因库登录，推测这些基因可能是与自然转移机制有关的分子，但未能获得转移的功能事件。

A metastasis from primary tumor is concluded through many processes. It is the important problem, which relates to the patient's prognosis closely and to make this analysis of the therapy for the cancer. We established the lung cancer cell line, which it only metastasis to the lung. Subtraction was carried out between metastasis and non-metastasis cells. We analyzed four genes, which related to the metastasis. Laminin receptor (LR) gene is specific receptor toward cell adhesion protein to laminins. It was proved that there was a loss of exson in a part of the gene that a code does a 67kDa molecule as an analysis. And, PAF-AH is acetylhydlase of the activated factor of the phospholipids (PAF), which produced of the platelets, which relates to the tumor metastasis. It is involved in the movement of the nerve cell, but the cancer metastasis isn't clear. Participation is suggested to the metastasis control due to inactivation of PAF due to the expression of this PAF-AH gene with the non-metastasis line. Furthermore, RACE method was enforced, and it got the gene of the extension to analyze two unknown genetic fragments. These genes are related to the melanoma, one of others existed in 14 chromosome, which relates to the uterine cervix carcinoma and the intestine mucous membrane protein, and agreement were entry of Gene bank with it recently The genes are presumed probably to be the molecule, which relates to the natural metastasis mechanism but couldn't get the functional events for metastasis.