Study on treatment of malignant glioma with reference to IORT using radiosensitaizers.

(1)观察顺铂对A172和T98G肿瘤细胞系的放射治疗效果。在单细胞凝胶电泳法(SCG)检测中，CDDP对肿瘤细胞的杀伤作用明显强于不加CDDP的肿瘤细胞。(2)为了评价恶性胶质瘤的放射敏感性，结合增殖细胞核抗原(PCNA)、MIB-1和P53的免疫反应，进行了SCG分析。对22例患者的结果进行统计学分析，并与临床结果进行比较：SCG法DNA损伤细胞(DNA损伤指数：DDI)在10.5%~37.2%之间，因此，较低比例的DDI导致肿瘤在初治后早期复发。P53、DDiS和肿瘤复发率在P53、DDis和肿瘤复发率显著高于年轻组(p&lt；0.01)。(3)临床分析27例恶性肿瘤…患者的放射治疗效果。更多的t胶质瘤。结果1年生存率为72.6%，2年生存率为35.3%。(4)分析高级别(3、4级)脑胶质瘤患者术中加用放射治疗(IORT)后的生存率与肿瘤免疫细胞化学变化的关系。在选择的54例高级别胶质瘤患者中，11例在最初治疗后长期存活超过5年。回顾分析与延长生存期相关的临床病理特征是：(1)癫痫发作，(2)最初CT扫描显示小肿块无增强，随后发展为强化的较大肿块，(3)肿瘤位于额叶和皮质表面附近，(4)IORT中20~30Gy射线照射(平均25.5Gy一次)至切除后的肿瘤床，(5)低的增殖细胞核抗原标记指数(LI)。另一方面，分析IORT治疗幕上复发性低级别胶质瘤的疗效。在38例经组织学证实的幕上低级别Glima患者的记录中，12例成人患者中，6例接受了IORT，2年生存率为83.3%，显著高于对照组。较少

(1) Effectiveness of CDDP on raidotherapy was experimentally investigated in A172 and T98G tumor cell line. In the evaluation of single cell gel electrophoresis (SCG) assay, irradiation with CDDP resulted in significantly stronger killing effect on the tumor cells than that without CDDP.(2) To evaluate the radiosensitivity of human malignant gliomas, SCG assay in addition to the evaluations of proliferating cell nuclear antigen(PCNA), MIB-1 and p53 immunoreactivity were undergone. Those result in 22 patients was statistically analyzed, compared with clinical outcome : DNA-damaged cells on SCG assay (DNA damage index : DDI) ranged from 10.5 to 37.2%, thus, lower percentage DDIs resulted in tumor recurrence in the earlier periods after the initial treatment. In patients with accumulation of p53, DDIs and tumor recurrence rate were significantly higher but lower in younger, respectively (p<0.01).(3) Effectiveness of CDDP on radiotherapy was clinically analyzed in 27 patients with malignan … More t gliomas. In the results, the survival rate was 72.6% in 1-year and 35.3% in 2-year.(4) Patients with high grade (grade 3 and 4) gliomas who were given additional intraoperative radiation therapy (IORT) were analyzed survival in relation to the immunocytochemical assessment of their tumors. Of 54 selected patients with high grade gliomas, 11 had long-term survival over 5 years after the initial treatment. The clinico-pathological features, analyzed retrospectively, that were associated with prolonged survival were : (1) epileptic seizures, (2) initial CT scans showing a small mass without contrast enhancement which developed into an enhancing larger mass on follow up, (3) tumor location which was frequently frontal and near the cortical surface, (4) 20-30Gy irradiation (mean 25.5Gy) in IORT delivered in one fraction to the post-resection tumor bed, and (5) a low PCNA labelling index (LI). On the other hand, effectiveness of IORT for recurrent supratentorial low grade glioma was analyzed. Of twelve adult patients in the records of 38 patients with histologically verified supratentorial low grade glima, six underwent IORT.The 2-year survival rate was 83.3% and significantly higher. Less