Study on treatment of malignant glioma with reference to IORT using radiosensitaizers.

放射增敏剂参照IORT治疗恶性胶质瘤的研究

• 批准号: 06671380
• 负责人: SAKAI Noboru
• 金额: $ 1.22万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671380/
• 关键词: IORT; SCG assay; gliomas; PCNA; MIB; P53; radiation therapy; CDDP; radiosensitivity; malignant glioma; MIB-1

(1) Effectiveness of CDDP on raidotherapy was experimentally investigated in A172 and T98G tumor cell line. In the evaluation of single cell gel electrophoresis (SCG) assay, irradiation with CDDP resulted in significantly stronger killing effect on the tumor cells than that without CDDP.(2) To evaluate the radiosensitivity of human malignant gliomas, SCG assay in addition to the evaluations of proliferating cell nuclear antigen(PCNA), MIB-1 and p53 immunoreactivity were undergone. Those result in 22 patients was statistically analyzed, compared with clinical outcome : DNA-damaged cells on SCG assay (DNA damage index : DDI) ranged from 10.5 to 37.2%, thus, lower percentage DDIs resulted in tumor recurrence in the earlier periods after the initial treatment. In patients with accumulation of p53, DDIs and tumor recurrence rate were significantly higher but lower in younger, respectively (p<0.01).(3) Effectiveness of CDDP on radiotherapy was clinically analyzed in 27 patients with malignan … More t gliomas. In the results, the survival rate was 72.6% in 1-year and 35.3% in 2-year.(4) Patients with high grade (grade 3 and 4) gliomas who were given additional intraoperative radiation therapy (IORT) were analyzed survival in relation to the immunocytochemical assessment of their tumors. Of 54 selected patients with high grade gliomas, 11 had long-term survival over 5 years after the initial treatment. The clinico-pathological features, analyzed retrospectively, that were associated with prolonged survival were : (1) epileptic seizures, (2) initial CT scans showing a small mass without contrast enhancement which developed into an enhancing larger mass on follow up, (3) tumor location which was frequently frontal and near the cortical surface, (4) 20-30Gy irradiation (mean 25.5Gy) in IORT delivered in one fraction to the post-resection tumor bed, and (5) a low PCNA labelling index (LI). On the other hand, effectiveness of IORT for recurrent supratentorial low grade glioma was analyzed. Of twelve adult patients in the records of 38 patients with histologically verified supratentorial low grade glima, six underwent IORT.The 2-year survival rate was 83.3% and significantly higher. Less

(1)用A172和T98 G肿瘤细胞系研究顺铂的放射治疗作用。在单细胞凝胶电泳（SCG）评价中，CDDP照射对肿瘤细胞的杀伤作用明显强于未照射。(2)为了评价恶性胶质瘤的放射敏感性，除增殖细胞核抗原（PCNA）、MIB-1和p53免疫反应性外，还进行了SCG测定。对22例患者的这些结果进行统计学分析，与临床结果相比：SCG测定的DNA损伤细胞（DNA损伤指数：DDI）范围为10.5%至37.2%，因此，较低百分比的DDI导致初始治疗后早期肿瘤复发。p53蛋白表达增高的患者，DDI和肿瘤复发率明显增高，而年轻患者的DDI和肿瘤复发率明显降低（p<0.01）。(3)本文对27例恶性肿瘤患者应用顺铂进行放疗的临床分析 ...更多信息 t神经胶质瘤。1年生存率为72.6%，2年生存率为35.3%。(4)对接受额外术中放疗（IORT）的高级别（3级和4级）胶质瘤患者的生存率进行分析，并与肿瘤的免疫细胞化学评估相关。在54例高级别胶质瘤患者中，11例在初始治疗后长期生存超过5年。回顾性分析与生存期延长相关的临床病理学特征为：（1）癫痫发作，（2）初始CT扫描显示无对比增强的小肿块，在随访时发展为增强的较大肿块，（3）肿瘤位置通常位于额叶和靠近皮质表面，（4）IORT中20- 30 Gy（平均25.5Gy）一次性照射切除后瘤床;（5）低PCNA标记指数（LI）。另一方面，分析了IORT治疗复发性幕上低级别胶质瘤的有效性。在38例经病理证实的幕上低级别胶质瘤中，12例成人患者中有6例接受了IORT治疗，2年生存率为83.3%，明显高于成人患者。少

项目成果

Akira Hara: "Remission of Recurrent Primary Intracranial Malignant Lymphoma after High-dose Intra-arterial Corticosteroid Administration and Intra-arterial Chemotherap," Neurologia medico-chirurgica. 34. 700-703 (1994)

Akira Hara：“高剂量动脉内皮质类固醇给药和动脉内化疗后复发性原发性颅内恶性淋巴瘤的缓解”，Neurologia medico-chirurgica。

Akira Hara, Yasuhiko Kaku, Yasuaki Nishimura, Noboru Sakai, Hiromu Yamada: "Remission of recurrent primary intracranial malignant lymphoma after high-dose intra-arterial corticosteroid administration and intra-arterial chemotherapy" Neurologia medico-chir

Akira Hara、Yasuhiko Kaku、Yasuaki Nishimura、Noboru Sakai、Hiromu Yamada：“高剂量动脉内皮质类固醇给药和动脉内化疗后复发性原发性颅内恶性淋巴瘤的缓解” Neurologia medico-chir

Hiroshi Kobayashi: "Clinical applications of single cell gel electrophoretic (SCG) assay in human malignant glioma." Acta Scholae Medicinalis University in Gifu. 44. 140-150 (1996)

Hiroshi Kobayashi：“单细胞凝胶电泳（SCG）测定在人类恶性神经胶质瘤中的临床应用。”

Noboru Sakai,et.al.: "Longer survival in high grade gliomas:intraoperative radiation therapy (IORT) and immunocy to chemical assessment of their growth potential." Journal of Clinical Neuroscience. 3. 46-51 (1996)

Noboru Sakai 等人：“高级别胶质瘤的更长生存期：术中放射治疗 (IORT) 和对其生长潜力的化学免疫评估。”

Akira Hara, Yasuaki Nishimura, Noboru Sakai, Hiromu Yamada, Shigeo Yanagawa: "Effectiveness of intraoperative radiation therapy for recurrent supratentorial low grade glioma" Journal of Neuro-Oncology. 25. 239-243 (1995)

Akira Hara、Yasuaki Nishimura、Noboru Sakai、Hiromu Yamada、Shigeo Yanakawa：“术中放射治疗复发性幕上低级别胶质瘤的有效性”神经肿瘤学杂志。