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Telomerase activity in brain tumors.

脑肿瘤中的端粒酶活性。

基本信息

批准号：
09671415
负责人：
SAKAI Noboru
金额：
$ 1.92万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Telomerase activity in brain tumors was analyzed. Telomerase activity was examined in surgically resected 41 brain tumors, A172 human glioblastoma cells, and C6 rat glioma cells by means of telomeric repeat amplification protocol (TRAP) assay. In the 41 tumors, the activity of telomerase was compared with histologic diagnosis, the MIB-1 proliferative cell index and patient's prognosis. In A 172 glioblastoma cells and C6 glioma cells, the changes in the telomerase activity of the cells were analyzed during differentiation induced by dibutyryl cyclic AMP, theophylline and retinoic acid. Telomerase activity in rat gliomas induced by ethylnitrosourea administration in pregnant rats was also examined.Twelve of 20 glioblastomas, 2 of 2 oligodendrogliomas and 3 of 3 metastatic brain tumors demonstrated telomerase activity. However, anaplastic astrocytoma, low grade astrocytoma and meningioma exhibited no activity. Although no clear relations were confirmed in glioblastoma between telomerase activity and the MIB-1 proliferative cell index, the telomerase activity tended to correlate with patient's prognosis. After the cell differentiation, the telomerase activities of A 172 glioblastoma cells and C6 glioma cells were strongly decreased. There were no different telomerase activities among growth stages of rat gliomas induced by ethylnitrosourea.These results suggest that telomerase activity may be an important marker of brain tumor malignancy and that functional inhibition of the activity may play a role during differentiation of glioma cells.

检测脑肿瘤组织中端粒酶活性。采用端粒重复序列扩增法（TRAP）检测了41例手术切除的脑肿瘤、A172人胶质母细胞瘤细胞和C6大鼠胶质瘤细胞的端粒酶活性。对41例肿瘤进行端粒酶活性检测，并与组织学诊断、MIB-1增殖细胞指数及患者预后进行比较。以A172胶质母细胞瘤细胞和C6胶质瘤细胞为研究对象，分析了二丁酰环磷酸腺苷、茶碱和维甲酸诱导分化过程中细胞端粒酶活性的变化。用乙基亚硝基脲诱发的孕鼠脑胶质瘤进行端粒酶活性检测，20例胶质母细胞瘤中12例、2例少突胶质细胞瘤中2例、3例脑转移瘤中3例端粒酶活性阳性。而间变性星形细胞瘤、低级别星形细胞瘤和脑膜瘤无活性。虽然胶质母细胞瘤中端粒酶活性与MIB-1增殖细胞指数之间没有明确的关系，但端粒酶活性往往与患者的预后相关。A172胶质母细胞瘤细胞和C6胶质瘤细胞分化后端粒酶活性明显降低。结果表明，不同生长阶段的胶质瘤细胞端粒酶活性无明显差异，提示端粒酶活性可能是脑肿瘤恶性程度的重要标志，其活性的功能抑制可能在胶质瘤细胞分化过程中起作用。