脳切片電気生理学的手法を用いた低酸素性組織半影(penumbra)の研究

脑切片电生理技术研究缺氧组织半暗带

• 批准号: 06671413
• 负责人: 平松 謙一郎
• 金额: $ 1.22万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671413/
• 关键词: Brain slice; hippo campus; hypoxia; EPSP; oxygen tension

砂ネズミの海馬スライスを通常の実験系(interface chambar)で整理的状態を維持し、CA 1領域でEPSPs(Excitatory Post Synaptic Potentials,興奮性後シナプス電位)の細胞外電位記録および組織酸素分圧を同時にモニターした。低酸素負荷前の酸素分圧を安定させた後、種々の低酸素負荷を最長20分間加え際のEPSPsの変動と、再酸素供給後のEPSPsの回復の程度を調べ、低酸素負荷によりEPSPsが消失する群としない群との二群間で、低酸素負荷条件の差異を検討した。低酸素負荷前の組織酸素分圧およびEPSPsの電位に有為差はなく、スライスの組織環境ならびにsynaptic functionはほぼ相同と思われた。低酸素負荷後EPSPs消失群の組織酸素分圧は平均87.0mmHgで、全例12分以内にEPSPsが消失し脱分極を呈し、再酸素供給後もEPSPsの回復がみられないものがあった。非消失群は194.6mmHgで、20分間の低酸素負荷中EPSPsの電圧は減少するが、消失する事はなく脱分極もみられず、再酸素供給により程度に差わあるもののEPSPsの回復が認められた。これら二群間の負荷時の酸素分圧値には明らかな有為差が認められたが、消失群の中に150.0mmHgと比較的高い酸素分圧値でEPSPsが消失したものがあり、逆に非消失群の中に165.0mmHgと比較的低い酸素分圧値でもEPSPsが消失しないものが存在したため、負荷後の酸素分圧の絶対値だけではなく、負荷前後の酸素分圧の減少率に着目したところ、消失群の減少率は32.6%で、非消失群は40.7%であり、明かな有為差が認められた。以上のことより、synaptic functionに与える低酸素負荷の影響に関して、負荷後の酸素分圧の絶対値とともに、負荷前後の酸素分圧の減少比も重要な因子と考えられた。

The hippocampus of sand production is usually maintained in an organized state (interface chambar), and extracellular potential recording and histamine partial pressure are simultaneously maintained in the field of CA1 EPSPs(Excitatory Post Synaptic Potentials). The change of EPSPs before and after stabilization of acid partial pressure before low acid load, the change of EPSPs during the increase of acid partial pressure for up to 20 minutes, the degree of recovery of EPSPs after re-acid supply, the change of EPSPs during the decrease of acid partial pressure before and after acid partial pressure, the difference of acid partial pressure between two groups, and the difference of acid partial pressure before and after acid partial pressure were discussed. The potential of the tissue components before the low acid load is different from that of the tissue environment, and the synaptic function is the same. The mean tissue pressure of EPSPs disappeared within 87.0 mmHg after low acid loading, and EPSPs disappeared within 12 minutes of depolarization in all cases. The recovery of EPSPs after re-acid loading was also observed. The voltage of EPSPs in the non-vanishing group decreased from 194.6mmHg to 20 minutes under low acid load. The voltage of EPSPs decreased from 194.6mmHg to 20 minutes under low acid load. The voltage of EPSPs decreased from 194.6mmHg to 194.6mmHg under low acid load. The voltage of EPSPs decreased from 194.6mmHg to 20 minutes under low acid load. The voltage of EPSPs decreased from 194.6mmHg to 194.6mmHg under low acid load. The acid component pressure value between the two groups under load is different from each other. The acid component pressure value between the two groups is different from each other. The acid component pressure between the two groups is different from each other. The acid component pressure between the two groups is different from each other. The reduction rate of acid component pressure before and after load was significantly higher than that of non-loss group by 32.6%, and that of non-loss group by 40.7%. The above factors are related to the effect of low acid load, the relative value of acid pressure after load, and the reduction ratio of acid pressure before and after load.

项目成果

平松謙一郎: "大脳皮質切片モデルを利用したNOS阻害剤の低酸素性神経細胞障害に対する保護" 脳循環代謝. 6. 74-74 (1994)

Kenichiro Hiramatsu：“使用大脑皮层切片模型保护 NOS 抑制剂免受缺氧神经元损伤”《大脑循环与代谢》，6. 74-74 (1994)。

徳永英守: "海馬スライスにおける低酸素負荷条件の差異によるシナプス機能の障害" 脳卒中. 16. (1995)

Hidemori Tokunaga：“海马切片缺氧负荷条件差异导致突触功能受损”16。（1995）

平松謙一郎: "虚血性脳血管障害の病態-脳切片を用いたアプローチ-" 北海道脳神経疾患研究所選認. 7. (1995)

平松健一郎：“缺血性脑血管疾病的病理学-使用脑切片的方法-”北海道神经病学研究所精选7。（1995）。