Pathomechanism of radicular pain in lumbar radiculopathy.

腰椎神经根病中神经根痛的病理机制。

• 批准号: 06671473
• 负责人: KAWAKAMI Mamoru
• 金额: $ 1.28万
• 依托单位: Wakayama Medical College, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671473/
• 关键词: Nucleus Pulposus; Anulus Fibrosus; Pain; Hyperalgesia; Phospholipase A2; Nitric Oxide; Mepacrine; L-NAME; L-NAME; pH; インターロイキン

Fat, nucleus pulposus (NP) and anulus fibrosus (AF) were allografted into the epidural space at L_6 level in the rat. Withdrawal response thresholds to mechanical stimuli and withdrawal response latencies to thermal stimuli on the tail postoperatively. Interleukin-1, PLA_2 and NOS were examined in the applied tissues using immunohistochemistry, NADPH-diaphorase histochemistry and in situ hybridization. In results, allografted fat did not produce hyperalgesia. Allografts of NP and NP+AF showed evidence of mechanical and thermal hyperalgesia, respectively. Although interleukin-1 was demonstrated in all applied tissues, PLA_2 was only observed around the applied NP and NP+AF.NOS was only markedly increased around the applied. The NP group (allografted NP placed on the sciatic nerve) and NP+AF group (NP+AF,respectively) were mede to evaluate the sensitivities to noxious stimuli following local sciatic nerve administration of L-NAME or mepacrine into the operated site. Only rats in the NP group showed evidence of mechanical hyperalgesia. Injection of L-NAME resulted in evidence of mechanical hyperalgesia in the NP+AF group. Mechanical hyperalgesia, which was produced in the NP group and after injection of L-NAME in the NP+AF group, both of which returned to normal after mepacrine injection. In conclusion, it appears that PLA_2 and NO play important but different roles in pathomechanisms of radicular pain in lumbar disc herniation.

将脂肪、髓核（NP）和纤维环（AF）移植于大鼠L_6水平硬膜外腔。术后尾部对机械刺激的退缩反应阈值和对热刺激的退缩反应阈值。应用免疫组织化学、NADPH黄递酶组织化学和原位杂交技术检测局部组织中IL-1、PLA_2和NOS的表达。结果表明，同种异体脂肪移植不产生痛觉过敏。NP和NP+AF的同种异体移植物分别显示出机械和热痛觉过敏的证据。虽然IL-1在所有应用组织中均被检测到，但PLA_2仅在应用NP和NP+AF周围观察到，NOS仅在应用NP周围显著增加。NP组（移植于坐骨神经上）和NP+AF组（分别为NP+AF组）分别在坐骨神经局部注射L-NAME或米帕林后，评价神经对伤害性刺激的敏感性。只有NP组的大鼠表现出机械性痛觉过敏的证据。注射L-NAME导致NP+AF组出现机械性痛觉过敏。NP组和NP+AF组注射L-NAME后均产生机械性痛觉过敏，注射米帕林后均恢复正常。结论：PLA_2和NO在腰椎间盘突出症神经根性疼痛的发病机制中起着重要而不同的作用。

项目成果

Mamoru Kawakami, Tetsuya Tamaki, Hiroshi Hashizume, James N.Weinstein, Stephen T Meller: "The role of phospholipase A2 and nitric oxide in pain related behavior produced by application of intervertebral disc to the sciatic nerve in the rat" Transactions o

Mamoru Kawakami、Tetsuya Tamaki、Hiroshi Hashizume、James N.Weinstein、Stephen T Meller：“磷脂酶 A2 和一氧化氮在大鼠坐骨神经应用椎间盘产生的疼痛相关行为中的作用”

川上守: "椎間板組織による疼痛発現に及ぼす一酸化窒素、phospholipase A2の役割" 日本整形外科学会雑誌. 69(8). S1443- (1995)

Mamoru Kawakami：“一氧化氮和磷脂酶 A2 对椎间盘组织引起的疼痛表达的作用”，日本骨科学会杂志 69(8)。

Mamoru Kawakami: "The role of phospholipase A2 and nitric oxide in pain related behavior produced by application of intervertebral disc to the sciatic nerve in the rat." Transaction of Orthopedic Research Society.43. 188- (1996)

Mamoru Kawakami：“磷脂酶 A2 和一氧化氮在大鼠坐骨神经椎间盘应用产生的疼痛相关行为中的作用。”

Kawakami M,Tamaki T,Weinstein J,Hashizume H,Nishi H,Meller S,Gebhart GF: "Disc materials produce pain-related behavior in the rat : The role of pH,immune response and chemicals" Tansactions of Orthopaedic Research Society. 20 (2). 666 (1995)

Kawakami M、Tamaki T、Weinstein J、Hashizume H、Nishi H、Meller S、Gebhart GF：“椎间盘材料在大鼠中产生疼痛相关行为：pH、免疫反应和化学物质的作用”骨科研究协会的交易。

川上 守: "腰部椎間板ヘルニアの疼痛発現機序-硬膜外髄核移植による知覚過敏-" 中部日本整形外科災害外科学会雑誌. 37. 1215-1216 (1994)

Mamoru Kawakami：“腰椎间盘突出症的疼痛表现机制 - 硬膜外髓核移植引起的过敏”日本中部整形外科和灾难外科学会杂志 37. 1215-1216 (1994)。