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Localization of mRNA of procollagen type I in supraspinatus tendons of incomplete- thickness tears by in situ hybridization

原位杂交在不完全厚度撕裂的冈上肌腱中定位 I 型原胶原 mRNA

基本信息

批准号：
06671485
负责人：
FUKUDA Hiroaki
金额：
$ 1.22万
依托单位：
TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

ObjectiveIncomplete-thickness tears of the rotator cuff are painful conditions which are commonly seen in those aged over 40 and often mistaken for the 'frozen shoulder'. In order to determine the theoretical basis for treatment options, conservative or surgical, the healing capacity of incomplete-thickness tears was examined by detecting mRNAs of procollagen * 1 type I in the partially torn tendons.Materials and MethodsTen torn supraspinatus tendons were obtained during operation which were 3 bursal-side, 3 intratendinous and 4 joint-side tears. These specimens were fixed in 10% buffered formalin and embedded in paraffin. The digoxigenin-labeled oligonucleotide probe (22 mer), which was specific to human procollagen alpha 1 type I mRNA, was used in our in situ hybridization protocols.ResultsThe signals of procollagen alpha 1 type I mRNA were detected in the tenocytes and undifferentiated mesenchymal cells of or in the vicinity of the stumps of the torn supraspinatus tendons, but not in the fibroblasts of the subacromial bursa. They were scarcely observed in the specimens without apparent trauma.ConclusionsSignals were detected in 8 cases out of 10 incomplete-thickness cuff tears. Even in the long-standing tears, the signals were detected. An intratendinous tearing is considered to exteed both proximally and distally. Spontaneous tendon healing is unlikely in incomplete-thickness cuff tears. An additinal study is contemplated to quantitate mRNA in order to be more objective.

肩袖不完全撕裂是一种疼痛的情况，常见于40岁以上的人，经常被误认为是“冻结肩”。为了确定治疗方案的理论依据，保守或手术，愈合能力的不完全厚度的眼泪进行了检查，通过检测mRNA的前胶原 * 1型I在部分撕裂tendons.Materials和Methods10撕裂冈上肌腱在手术过程中获得3囊侧，3 intradinous和4关节侧撕裂。将这些标本固定在10%福尔马林缓冲液中，并包埋在石蜡中。地高辛标记的寡核苷酸探针（22聚体），这是特定的人前胶原α 1型I mRNA，被用于在我们的原位杂交protocols.ResultsThe信号的前胶原α 1型I mRNA检测肌腱细胞和未分化的间充质细胞或附近的残端撕裂冈上肌腱，但不是在成纤维细胞的肩峰下囊。他们几乎没有观察到的标本没有明显的traums.ConclusionsSignals检测在8例10不完全厚度袖带撕裂。即使在长期的眼泪，信号被检测到。腱内撕裂被认为是近端和远端的延伸。不完全厚度的肩袖撕裂不太可能发生肌腱自发愈合。为了更客观，考虑进行额外研究以定量mRNA。