The effect of TNF- alpha on the fibroblast of proliferative scar

TNF-α对增殖性疤痕成纤维细胞的影响

• 批准号: 06671497
• 负责人: HAMANAKA Takaomi
• 金额: $ 1.15万
• 依托单位: KAWASAKI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671497/
• 关键词: proliferative scar; keloid; hypertrophic scar; TNF-alpha; IFN alpha-2b; cell culture; collagen gel; 成熟瘢痕; IFNα-2b; ELISA

Tumor Necrosis Factor (TNF-alpha) or Cachectin has been implcated as a mediator of many of the adverse host responses to injury and infection. TNF has been shown to be elevated in the serum of burn patients during the acute phase of burn injury. Also, the level of these elevations contributes to delayd wound healing in such patients. In this study we evaluated the serum TNF-alpha levels of burn patients and the patients of keloid and hypertrophic scar to ascertain whether elevated levels of this mediator persists in these patients, leading to compromised wound healing.Our results indicate that TNF-alpha is significantly elevated in patients with major burns, up to 5 years after the injury. This may pose a contributing factor towards the increased rate of infections and delayd wound healing seen in these patients. We are presently, prospectively looking at TNF levels and scar formation, as there seems to be a yin-yang relationship between TNF and scarring. Studies suggest that TNF in low concentrations helps wound healing, whereas higher concentrations may be detrimental.

肿瘤坏死因子（TNF-α）或Cachectin已被认为是许多宿主对损伤和感染的不良反应的介质。已显示在烧伤急性期烧伤患者血清中TNF升高。此外，这些升高的水平有助于延迟此类患者的伤口愈合。在这项研究中，我们评估了烧伤患者和瘢痕疙瘩和增生性瘢痕患者的血清TNF-α水平，以确定是否在这些患者中这种介质的水平升高持续存在，从而导致伤口愈合受损。我们的研究结果表明，TNF-α在严重烧伤患者中显着升高，最多5年后受伤。这可能是导致这些患者感染率增加和伤口愈合延迟的一个因素。我们目前正在前瞻性地研究TNF水平和瘢痕形成，因为TNF和瘢痕形成之间似乎存在阴阳关系。研究表明，低浓度的TNF有助于伤口愈合，而高浓度可能是有害的。

项目成果

Sahara, K.: "The effect of GM-CSF on the inhibition of contraction of excisional wounds caused by bacterial cantamination." Kawasaki MED. J.20. 87-98 (1994)

Sahara, K.：“GM-CSF 对抑制细菌污染引起的切除伤口收缩的作用。”

浜中孝臣: "顔面外傷のプライマリ・ケア" 外科. 57. 710-714 (1995)

Takaomi Hamanaka：“面部创伤的初级护理”手术 57. 710-714 (1995)。

Hamanaka, Takaomi: "Primary care of facial injures." Surgery. 57. 711-714 (1995)

滨中高臣：“面部损伤的初级护理。”

Sahara, Keiichiro et al.: "The effect of GM-CSF on the inhibition of contraction of excisional wounds caused by bacterial contamination." Kawasaki MED.J.20. 87-98 (1994)

Sahara, Keiichiro 等人：“GM-CSF 对抑制细菌污染引起的切除伤口收缩的作用。”

Sahara,K.: "Persistent elevation of TNF-α in burn patients may contribute to compromised wound healing." Kawasaki MED.J.20. 79-85 (1994)

Sahara, K.：“烧伤患者中 TNF-α 的持续升高可能会导致伤口愈合受损。” Kawasaki MED.J.20 (1994)。