ISOLATION AND CHARACTERIZATION OF A NOVEL HUMAN SPERM ANTIGEN (H-SP-1)
新型人类精子抗原 (H-SP-1) 的分离和表征
基本信息
- 批准号:06671697
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We isolated a human sperm antigen gene (h-sp-1) by screening from human testis cDNA library with human sperm specific antisera. The sequence analysis of the h-sp-1 gene indicated that it contained 2170 bp and had 45% homology to human synaptophysin gene, which was considered to play a crucial role in calcium dependent signal transduction in synaptic vesicles in the terminal end of nerves. The analysis of the deduced amino acids from the h-sp-1 gene showed that it encoded 259 amino acids containing four hydrophilic regions and four hydrophobic regions. This indicated that the molecules encoded by the h-sp-1 gene might be a transmembrane protein with four membrane spanning regions like the synaptophysin. In order to characterize the molecules encoded by the hsp-1 gene, the antisera were generated by immunizing rabbits and mice with synthetic polypeptides (No174-198) based on the h-sp-1 gene. The resultant antisera stained fixed human sperm head by indirect immunofluorescence staining. The purified anti-h-sp-1 antibodies showed the inhibitory effect on human sperm penetration assay into zona-free hamster eggs. However they did not show any sperm immobilizing and agglutinating activities. These results suggested that the molecules encoded by the h-sp-1 gene might play an important role in the sperm-egg interaction.
用人精子特异性抗血清从人睾丸cDNA文库中筛选出一个人精子抗原基因(h-sp-1)。序列分析表明,h-sp-1基因全长2170 bp,与人突触素基因同源性为45%,该基因被认为在神经末梢突触囊泡的钙依赖性信号转导中起重要作用。对h-sp-1基因推导的氨基酸序列进行分析,结果表明该基因编码259个氨基酸,含有4个亲水区和4个疏水区。这表明h-sp-1基因编码的分子可能与突触素一样,是一种具有四个跨膜区的跨膜蛋白。为了表征hsp-1基因编码的分子,通过用基于hsp-1基因的合成多肽(No174-198)免疫兔和小鼠来产生抗血清。用间接免疫荧光染色法对固定的人精子头部进行染色。纯化的抗h-sp-1抗体对人精子穿透去透明带金黄地鼠卵的实验有抑制作用。然而,它们没有表现出任何精子固定和凝集活性。这些结果表明,h-sp-1基因编码的分子可能在精卵相互作用中起重要作用。
项目成果
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KOMORI Shinji其他文献
KOMORI Shinji的其他文献
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{{ truncateString('KOMORI Shinji', 18)}}的其他基金
Study for the function of TCTP in spermatogenesis and analysis for the regulation of the expression of TCTP by androgen.
TCTP在精子发生中的功能研究及雄激素对TCTP表达调控的分析。
- 批准号:
18591817 - 财政年份:2006
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
ANALYSIS FOR ANDROGENIC EFFECT IN BONE HETABOLIIM
骨代谢的雄激素作用分析
- 批准号:
13671757 - 财政年份:2001
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
ANALYSIS FOR THE ROLE OF HUMAN SPERM ACROSOMAL MOLECULEH-SP-1 IN SIGNAL TRANSDUCTION VIA CALCIUM CHANNEL
人精子顶体分子H-SP-1在钙通道信号转导中的作用分析
- 批准号:
11671661 - 财政年份:1999
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MOLECULAR ANALYSIS OF HUMAN SPERM ACROSOMAL MOLECULE H-SP-1 IN SPERM-EGG INTERACTION
人精子顶体分子 H-SP-1 在精卵相互作用中的分子分析
- 批准号:
09671723 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MOLECULAR ANALYSIS FOR HUMAN ANDROGEN RECEPTOR GENE IN CASES WITH ANDROGEN INSENSITIVITY SYNDROME
雄激素不敏感综合征患者雄激素受体基因的分子分析
- 批准号:
04671401 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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Postmortem genetic analysis for calcium-dependent signal transduction molecules in sudden unexplained death cases
不明原因猝死病例中钙依赖性信号转导分子的死后遗传分析
- 批准号:
20590691 - 财政年份:2008
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