ANALYSIS FOR ANDROGENIC EFFECT IN BONE HETABOLIIM

骨代谢的雄激素作用分析

• 批准号: 13671757
• 负责人: KOMORI Shinji
• 金额: $ 2.24万
• 依托单位: HYOGO COLLEGE OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671757/
• 关键词: androgen; androgen receptor; bone metabolism; osteoblast; osteosarcoma; transformation; 骨肉腫細胞株; 骨粗鬆症

Bone metabolism are affected by various factors such as hormone, cytokines mineral etc. Among them, hormones play an important role in bone metabolism. Estrogen is a very important hormone related to bone metabolism and a lot of studies for the role of estrogen have been reported. On the other hand, androgen seems to be also an important factor in bone metabolism. However, it is not enough to investigate the androgenic effect in bone metabolism. In this study, we analyzed the androgenic effect in human androgen receptor positive osteosarcoma cell line, which has ability to bone formation. In this cell line, dihydrotestosterone (DHT) did not induce the bone formation, whereas estradiol and bone morphogenic protein 2(BMP 2) showed the induction or bone formation. DHT suppressed the effect of estradiol and BHP 2 in bone formation. Moreover, DHT also suppressed the expression of osteoprotegrin which suppressed the development of osteoclast. In summary, androgen shows the suppressive effect in bone formation by interfering the effect of estradiol and BMP 2 and inducible effect in bone absorption by the suppression of the expression of osteoprotegrin. Androgen play an important role in bone metabolism.

骨代谢受激素、细胞因子、矿物质等多种因素的影响，其中激素在骨代谢中起着重要作用。雌激素是一种与骨代谢相关的重要激素，国内外对雌激素的作用进行了大量的研究。另一方面，雄激素似乎也是骨代谢的重要因素。然而，研究雄激素在骨代谢中的作用还不够。本研究分析了人雄激素受体阳性骨肉瘤细胞系的雄激素效应，该细胞系具有成骨能力。在该细胞系中，双氢睾酮（DHT）不诱导骨形成，而雌二醇和骨形态发生蛋白2（BMP 2）显示出诱导或骨形成。双氢睾酮可抑制雌二醇和BHP-2对骨形成的作用。此外，双氢睾酮还抑制了骨保护素的表达，从而抑制了破骨细胞的发育。总之，雄激素通过干扰雌二醇和BMP 2的作用而显示出骨形成的抑制作用，并且通过抑制骨保护素的表达而显示出骨吸收的诱导作用。雄激素在骨代谢中起重要作用。