ANALYSIS FOR ANDROGENIC EFFECT IN BONE HETABOLIIM

骨代谢受激素、细胞因子、矿物质等多种因素的影响，其中激素在骨代谢中起着重要作用。雌激素是与骨代谢相关的一种非常重要的激素，已有大量关于雌激素作用的研究报道。另一方面，雄激素似乎也是骨代谢的一个重要因素。然而，对雄激素在骨代谢中的作用进行研究还不够。在本研究中，我们分析了具有成骨能力的人雄激素受体阳性骨肉瘤细胞系的雄激素作用。在该细胞系中，双氢睾酮(DHT)不诱导成骨，而雌二醇和骨形态发生蛋白2(BMP-2)有诱导或成骨作用。DHT可抑制雌二醇和BHP-2的成骨作用。此外，DHT还抑制了破骨细胞的发育，从而抑制了骨整合蛋白的表达。综上所述，雄激素通过干扰雌二醇和BMP-2的作用而抑制骨形成，通过抑制骨整合蛋白的表达而诱导骨吸收。雄激素在骨代谢中起着重要作用。

Bone metabolism are affected by various factors such as hormone, cytokines mineral etc. Among them, hormones play an important role in bone metabolism. Estrogen is a very important hormone related to bone metabolism and a lot of studies for the role of estrogen have been reported. On the other hand, androgen seems to be also an important factor in bone metabolism. However, it is not enough to investigate the androgenic effect in bone metabolism. In this study, we analyzed the androgenic effect in human androgen receptor positive osteosarcoma cell line, which has ability to bone formation. In this cell line, dihydrotestosterone (DHT) did not induce the bone formation, whereas estradiol and bone morphogenic protein 2(BMP 2) showed the induction or bone formation. DHT suppressed the effect of estradiol and BHP 2 in bone formation. Moreover, DHT also suppressed the expression of osteoprotegrin which suppressed the development of osteoclast. In summary, androgen shows the suppressive effect in bone formation by interfering the effect of estradiol and BMP 2 and inducible effect in bone absorption by the suppression of the expression of osteoprotegrin. Androgen play an important role in bone metabolism.