Discharge of collagenase from a gingival fibroblast infected with oral mycoplasma

口腔支原体感染牙龈成纤维细胞胶原酶的排出

• 批准号: 06671801
• 负责人: KIKUCHI Hiroko
• 金额: $ 0.38万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671801/
• 关键词: Oral Mycoplasma; Collagenase; Fibroblast; IL-6; マイコプラズマ感染; 誘導; コラゲナーゼ放出

Human normal gingival fibroblast (Gin-1) cells cultured in a plate showed a morphological change to spheroid with time when they were exposed to either vialbe cells of Mycoplasma salivarium (Ms) or Mycoplasma orale (Mo) or cell membranes of Ms or Porphyromonas gingivalis (Pg) cells fractured by sonication. This morphological change was most strongly induced by viable cells of Mo and Ms membranes. The latter further caused, after 96 h-exposure, detachment of Gin-1 cells from the plate.The morphological change and the detachment were supposed to be caused by collagenase discharged from the cells, but no collagenase activity was found in the culture medium. There is another possibility that the morphological change and the detachment are attributed to cytokines produced and, hence, the production of inflammatory cytokines (IL-1beta, IL-6 and TNFalpha) in the culture medium was investigated by using an enzyme-linked immunosorvent assay. High contents of IL-6 were detected for all cases. TNFalpha and IL-1beta were also detected for Pg stimulation but not for Ms and Mo stimulations.IL-6 was originally identified as a factor which stimulates immunoglobulin production by B cells and is discussed to be involved in inflammation such as arthritis. In the field of dental science, IL-6 was found in the lesion of periodontal disease and is discussed to be involved in its etiology. Therefore, the present finding that oral mycoplasmas induce IL-6 secretion from a gingival fibroblast suggests that they play an important etiological role in periodontal diesease.

人正常牙龈成纤维细胞（Gin-1）经唾液支原体（Ms）或口腔支原体（Mo）培养，或经超声破碎Ms或牙龈卟啉单胞菌（Pg）细胞膜后，细胞形态发生变化，随时间延长逐渐变成球状体。这种形态变化是最强烈的诱导钼和Ms膜的活细胞。96 h后，Gin-1细胞从培养板上脱落，其形态学变化和脱落可能是由细胞分泌胶原酶引起的，但培养液中未发现胶原酶活性。存在另一种可能性，即形态变化和脱离归因于产生的细胞因子，因此，通过使用酶联免疫吸附测定研究了培养基中炎性细胞因子（IL-1 β、IL-6和TNF α）的产生。所有病例血清IL-6含量均较高。TNF α和IL-1 β也被检测到用于Pg刺激，但不用于Ms和Mo刺激。IL-6最初被鉴定为刺激B细胞产生免疫球蛋白的因子，并被讨论为参与炎症如关节炎。在口腔科学领域，IL-6被发现存在于牙周病的病变中，并被认为与牙周病的病因有关。因此，目前发现口腔支原体诱导牙龈成纤维细胞分泌IL-6，表明它们在牙周疾病中发挥重要的病因作用。