Apoptosis of immuno-competent cells induced by oral mycoplasmas

口腔支原体诱导免疫活性细胞凋亡

• 批准号: 08672148
• 负责人: KIKUCHI Hiroko
• 金额: $ 1.28万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672148/
• 关键词: Oral mycoplasmas; T cells; concanavalin A; apoptosis; TNFalpha; IL-6; IL-8; PCR; マイコプラズマ; 歯肉線維芽細胞; TNF-α

Oral mycoplasmas, Mycoplasma salivarium and Mycoplasma fermentans possessed policlonal mitogens for mouse splenic T cells. In addition, they possessed the activity of enhancing concanavalin A-induced apoptosis of mouse splenic T cells and tumor necrosis factor-alpha playd an important role in expression of the activity.Mycoplasma salivarium and Mycoplasma fermentans possessed the activity to induce interleukin-6 and interleukin-8 in human gingival fibroblasts.A polymerase chain reaction assay for Mycoplasma salivarium by using the nucleotide sequence within aminopeptidase My gene was developed, which enabled rapid and specific detection of Mycoplasma salivarium in clinical samples.

口腔支原体、唾液支原体和发酵支原体对小鼠脾T细胞具有多克隆有丝分裂原。此外，本发明还提供了一种方法，它们具有增强刀豆球蛋白A诱导的小鼠脾T细胞凋亡的活性，肿瘤坏死因子-α在该活性的表达中起重要作用;唾液支原体和发酵支原体具有诱导白细胞介素-6和白细胞介素-6的活性，8的人牙龈成纤维细胞。聚合酶链反应检测唾液支原体，通过使用氨肽酶My基因内的核苷酸序列，该技术能够快速、特异地检测临床样本中的唾液支原体。

项目成果

Ken-ichiro Shibata et al.: "Mycoplasma salivarium induce interleukin-6 and interleukin-8 in human gingival fibroblasts" FEMS lmmunology and Medical Microbiology. 19. 275-283 (1998)

Ken-ichiro Shibata 等人：“唾液支原体在人牙龈成纤维细胞中诱导白细胞介素 6 和白细胞介素 8”FEMS 免疫学和医学微生物学。

申川 哲也、他6名: "Development of a polymerase chain reaction assay for Mycoplasma salivarium by using the nucliofide seguence within aminopeptidore My gerl." FEMS Microbiology Letters. 156. 281-286 (1997)

Tetsuya Shinkawa，其他 6 人：“利用氨基肽内的核苷酸序列开发唾液支原体的聚合酶链反应测定 My gerl”，FEMS 微生物学快报 156. 281-286 (1997)。