REGENERATED TASTE BUDS AND SALT TASTE RESPONSES IN THE RAT CHORDA TYMPANI NERVE

大鼠鼓索神经再生的味蕾和盐味反应

• 批准号: 06671855
• 负责人: KITADA Yasuyuki
• 金额: $ 1.09万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671855/
• 关键词: Rat; Regenerated taste bud; Regenerated chorda tympani nerve; Amiloride; アミロライド; 再生鼓索神経

After chorda tympani nerve (CT) transection, taste buds degenerate and disappear. Given time, however, taste buds reappear in fungiform papillae after regeneration of the cut nerve. In the present study, recovery of Na salts response from the regenerated CT after unilateral transection of the chorda tympanilingual nerve in the adult rats was investigated using amiloride, a sodium ion channel blocker. Taste responses from the regenerated CT were first obtained 28 days after transection of the nerve. Ratios for all responses were calculated relative to the 0.5 M NH_4CI response. The relative magnitude of the responses to 0.3-0.5 M NaCl and Na acetate from the regenerated CT were about 0.6 on days 28-35, and were about 0.8 on days 36-45. Results of amiloride treatment revealed that amiloride-sensitive components in the regenerated CT was small on days 28-35 and abruptly increase thereafter. However, amiloride-insensitive components were not related to times after transection of the nerve, when the magnitude of the responses to Na salts was expressed relative to that to 0.5 M NH_4Cl. La ions, a tight junction blocker, at 2.5 mM did not affect amilorideinsensitive components in both the regenerated CT in surgery rats and the intact CT in control rats. This suggests that paracellular pathways between taste cells are not involved in amiloride-insensitive sodium taste reception in the rat.

鼓索神经切断后，味蕾变性消失。然而，假以时日，切断的神经再生后，味蕾会重新出现在菌状乳头中。本研究用钠离子通道阻滞剂阿米洛利观察成年大鼠一侧鼓索神经切断后再生的CT对钠盐反应的恢复。再生的CT在神经切断28天后首次获得味觉反应。计算了所有反应相对于0.5M NH_4CI反应的比率。再生CT对0.3-0.5M氯化钠和醋酸钠的反应在第28-35天约为0.6，在36-45天约为0.8。阿米洛利治疗的结果显示，在28-35天，再生CT中对阿米洛利敏感的成分很少，此后突然增加。然而，阿米洛利不敏感成分与神经切断后的时间无关，即对钠盐的反应比对0.5MNH_4CL的反应大小。紧密连接阻滞剂La~(2+)在2.5 mM对手术大鼠再生CT和正常大鼠完整CT的不敏感成分均无影响。这表明味觉细胞之间的旁细胞通路不参与大鼠对阿米洛利不敏感的钠味觉接受。

项目成果

Y.Kitada and Y.Mitoh: "Responses to salts in the chorda tympani nerve of the rat : effects of amiloride and La ions on responses to Na salts in the regenerated nerve after nerve transection." Jpn.J.Taste Smell Res.Vol.2(3). S193-S196 (1995)

Y.Kitada 和 Y.Mitoh：“大鼠鼓索神经中盐的反应：阿米洛利和 La 离子对神经横断后再生神经中钠盐反应的影响。”

北田泰之,美籐純弘: "ラット鼓索神経の塩応答:再生神経の塩応答に対するアミロライドおよびランタンイオンの効果" 日本味と匂学会誌. 2. S193-S196 (1995)

Yasuyuki Kitada、Sumihiro Mito：“大鼠鼓索神经的盐反应：阿米洛利和镧离子对再生神经的盐反应的影响”日本味觉学会杂志 2. S193-S196 (1995)。

北田泰之,美藤純弘: "ラット鼓索神経の塩応答:再生神経の塩応答に対するアミロライドおよびランタンイオンの効果" 日本味と匂学会誌. 2. S193-S196 (1995)

Yasuyuki Kitada、Sumihiro Bito：“大鼠鼓索神经的盐反应：阿米洛利和镧离子对再生神经的盐反应的影响”日本味觉学会杂志 2. S193-S196 (1995)。