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Intercellular signaling in frog taste buds: taste cells and basal cells

青蛙味蕾的细胞间信号传导：味觉细胞和基底细胞

基本信息

批准号：
10671745
负责人：
KITADA Yasuyuki
金额：
$ 2.11万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671745/
关键词：
taste taste bud basal cell whole-cell path-clamp technique whole-cell current (6) frog frog 電気生理学シグナル伝達茸状乳頭

项目摘要

There is morphological evidence for synaptic interactions between taste cells and basal cells in vertebrate taste buds. However, very little is known about a role of basal cells in the processing of gustatory signals. Membrane properties of cells at the basal region of a taste disk were investigated in this study. Firstly, we found that the apical membrane receptor sites are responsible for the taste responses of the frog glossopharyngeal nerve to electrolytes. This implies that small molecules like Naions do not use paracellular pathways. Therefore, excitation of basal cells would be elicited only by that of taste receptor cells. Secondanly, we investigated the membrane properties of cells by applying the patch-clamp technique to cells isolated from frog taste organ. Some of isolated rod cells and wing cells possess the two processes to the apical and to the basal region. Voltage-gated NaィイD1+ィエD1 inward currents and KィイD1+ィエD1outward currents were recorded in both rod cells and wing … More cells. The patch-clamp technique was also applied to 25 cells at the basal region of ataste organ in a slice of lingual epithelium. Ratios, voltage-gated NaィイD1+ィエD1 inward current/ KィイD1+ィエD1 outward current, varied with each cell. Only KィイD1+ィエD1 outward current (lack of NaィイD1+ィエD1 inward current) and large NaィイD1+ィエD1 inward current/small K+ outward current were seen in 6 cells (mean ratio ± SE, 0.17 ± 0.05) and 5 cells (12.97 ± 0.4.77), respectively. Other 14 cells exhibited large NaィイD1+]ィエD1 inward current/large KィイD1+ィエD1 outward current (1.82 ± 0.19). It appears that there are different types of cells at the basal region of taste organ. Among 9 cells at the basal region, application of 100 μM 5-HT to the Ringer solution led to an increase of input membrane resistance in 3 cells and a decrease in 2 cells. Since basal cells like Merkel cells contain 5-HT, the changes in input membrane resistance by 5-HT suggest that basal cells may modulate gustatory signals at the basal region of taste organ. Less

有形态学证据表明，味觉细胞和基底细胞之间的突触相互作用在脊椎动物的味蕾。然而，我们对基底细胞在味觉信号处理中的作用知之甚少。本文研究了味觉盘基底区细胞的膜特性。首先，我们发现顶端膜受体位点负责蛙舌咽神经对电解质的味觉反应。这意味着像Naions这样的小分子不使用细胞旁通路。因此，基底细胞的兴奋只能由味觉受体细胞的兴奋引起。其次，应用膜片钳技术对青蛙味觉细胞进行膜特性研究。部分分离的杆状细胞和翅状细胞具有向顶端和向基部两个突起。杆状细胞和翅状细胞均记录到电压门控的Na γ γ γ D1+ γ γ γ D1向内电流和K γ γ D1+ γ γ γ D1向外电流。膜片钳技术也应用于舌上皮切片上味觉器官基底区的25个细胞。电压门控的Na γ γ γ D1+ γ γ γ D1向内电流/ K γ γ D1+ γ γ γ D1向外电流的比值随细胞的变化而变化。6个细胞（平均比值±SE， 0.17±0.05）和5个细胞（平均比值12.97±0.4.77）仅见K+ D1+ D1外向电流（缺乏Na + D1+ D1内向电流）和Na + D1+ D1内向电流大/ K+ D1外向电流小。另有14个细胞表现出较大的Na γ γ γ + γ γ γ γ向内电流/较大的K γ γ + γ γ向外电流（1.82±0.19）。感觉器官基底区有不同类型的细胞。在基底区9个细胞中，100 μM 5-HT作用于林格溶液后，3个细胞的输入膜电阻增加，2个细胞的输入膜电阻降低。由于梅克尔细胞等基底细胞含有5-羟色胺，因此5-羟色胺对输入膜阻力的改变提示基底细胞可能在味觉器官基底区调节味觉信号。少